SINGLE CRYSTAL XAS OF CUSF

CUSF 的单晶 XAS

• 批准号: 8170051
• 负责人: James E. Penner-Hahn
• 金额: $ 0.34万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170051
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Disease; Funding; Grant; Institution; Measurement; Metal Binding Site; Metals; Proteins; Research; Research Personnel; Resolution; Resources; Site; Solutions; Source; Structure; United States National Institutes of Health; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Initial crystallographic measurements suggest an unprecedented metal binding site in the Cu efflux protein CusF, with a novel organometallic structure. However, the crystal has modest resolution and more importantly is disordered near the metal, with only partial metal-site occupancy. A combination of solution and single crystal XAS will be used to provide detailed local structural information for the apparently unprecedented metal site.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 初步晶体学测量表明 Cu 流出蛋白 CusF 中存在前所未有的金属结合位点，具有新颖的有机金属结构。然而，晶体的分辨率适中，更重要的是，在金属附近是无序的，仅占据部分金属位点。溶液和单晶 XAS 的组合将用于为显然前所未有的金属位点提供详细的局部结构信息。