STUDIES OF METALLOPROTEINS (PFL ACTIVATING ENZYME, HPPE, NIKR, SYRB2)

金属蛋白的研究（PFL 激活酶、HPPE、NIKR、SYRB2）

• 批准号: 7721847
• 负责人: JESSICA VEY
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721847
• 关键词: Acids; Anabolism; Biological Factors; Cell physiology; Computer Retrieval of Information on Scientific Projects Database; Crystallography; DNA-Binding Proteins; Enzymes; Funding; Grant; Institution; Iron; Laboratories; Metabolism; Metalloproteins; Metals; Mononuclear; Nickel; Protein Binding; Proteins; Research; Research Personnel; Resources; Site; Source; Structure; United States National Institutes of Health; epoxidase; formate C-acetyltransferase; formate acetyltransferase activating enzyme; genetic regulatory protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Metals and metalloproteins are essential for many cellular processes. Our laboratory uses macromolecular crystallography to study the structure and function of metalloproteins. These proteins include nickel regulatory protein NikR, a DNA-binding protein; pyruvate-formate lyase activating enzyme (PFL-AE), a Radical SAM enzyme involved in anaerobic metabolism by formation of a protein bound glycyl radical on pyruvate formate lyase; hydroxypropylphosphonic acid epoxidase (HppE), which uses a mononuclear iron site to catalyze a unique epoxidation; and SyrB2, a mononuclear iron protein involved in natural product biosynthesis.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 金属和金属蛋白对于许多细胞过程至关重要。 我们实验室利用大分子晶体学来研究金属蛋白的结构和功能。 这些蛋白质包括镍调节蛋白 NikR，一种 DNA 结合蛋白；丙酮酸甲酸裂解酶激活酶（PFL-AE），一种自由基 SAM 酶，通过在丙酮酸甲酸裂解酶上形成蛋白质结合的甘氨酰自由基来参与无氧代谢；羟丙基膦酸环氧化酶（HppE），利用单核铁位点催化独特的环氧化反应； SyrB2，一种参与天然产物生物合成的单核铁蛋白。