CRYSTALLOGRAPHIC STUDIES OF REBC, PFL ACTIVATING ENZYME, AND MEAB

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们的实验室使用大分子结晶学来研究感兴趣的蛋白质和酶的结构和功能。这些蛋白质包括RebC，一种参与天然产物生物合成的酶，已知具有抗肿瘤活性；PflAE，一种自由基SAM酶；以及MeaB，一种与药物相关的GTP酶。这些酶的结构都是最近在我们的实验室里解决的(所有手稿都在准备中，结果还没有发表)，需要重要的后续实验。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our laboratory uses macromolecular crystallography to study the structure and function of proteins and enzymes of interest. These proteins include RebC, an enzyme involved in the biosynthesis of a natural product know to have antitumor activity; PflAE, a Radical SAM enzyme; and MeaB, a medicinally relevant GTPase. The structures of these enzymes have all been solved very recently in our lab (all manuscripts in preparation and unpublished results), and require important follow-up experiments.