STUDIES OF METALLOPROTEINS (PFL ACTIVATING ENZYME, HPPE, NIKR, SYRB2)

金属蛋白的研究（PFL 激活酶、HPPE、NIKR、SYRB2）

• 批准号: 7598060
• 负责人: JESSICA VEY
• 金额: $ 0.5万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598060
• 关键词: Acids; Anabolism; Biological Factors; Cell physiology; Computer Retrieval of Information on Scientific Projects Database; Crystallography; DNA-Binding Proteins; Enzymes; Funding; Grant; Institution; Iron; Laboratories; Metabolism; Metalloproteins; Metals; Mononuclear; Nickel; Protein Binding; Proteins; Research; Research Personnel; Resources; Site; Source; Structure; United States National Institutes of Health; epoxidase; formate C-acetyltransferase; formate acetyltransferase activating enzyme; genetic regulatory protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Metals and metalloproteins are essential for many cellular processes. Our laboratory uses macromolecular crystallography to study the structure and function of metalloproteins. These proteins include nickel regulatory protein NikR, a DNA-binding protein; pyruvate-formate lyase activating enzyme (PFL-AE), a Radical SAM enzyme involved in anaerobic metabolism by formation of a protein bound glycyl radical on pyruvate formate lyase; hydroxypropylphosphonic acid epoxidase (HppE), which uses a mononuclear iron site to catalyze a unique epoxidation; and SyrB2, a mononuclear iron protein involved in natural product biosynthesis.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 金属和金属蛋白是许多细胞过程所必需的。我们实验室使用大分子结晶学来研究金属蛋白的结构和功能。这些蛋白质包括镍调节蛋白NikR，一种DNA结合蛋白；丙酮酸-甲酸裂解酶激活酶(PFL-AE)，一种自由基SAM酶，通过在丙酮酸甲酸裂解酶上形成蛋白结合的甘氨酸基参与无氧代谢；羟丙基膦酸环氧化物酶(HppE)，利用单核铁中心催化独特的环氧化反应；以及SyrB2，参与天然产物生物合成的单核铁蛋白。