GENOMIC AND PROTEOMIC ANALYSIS OF PHI32, A NOVEL ESCHERICHIA COLI PHAGE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在大规模筛选可用于治疗牛乳房炎的噬菌体的过程中，分离到一种新的感染大肠杆菌的噬菌体。对该噬菌体的77,554bp基因组进行了测序和注释，并用电子显微镜和蛋白质组学方法对其病毒粒子进行了鉴定。鉴定了两种与宿主RNA聚合酶相互作用的phi32编码蛋白。其中一个是ECF家族的S因子，它可能与某些病毒基因的转录有关。另一种RNA聚合酶结合蛋白是一种新的转录抑制因子，其作用机制尚不清楚。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A novel phage infecting Escherichia coli was isolated during a large-scale screen for phages that may be used for therapy of mastitis in cattle. The 77,554 bp genome of the phage, named phi32, was sequenced and annotated, and its virions were characterized by electron microscopy and proteomics. Two phi32-encoded proteins that interact with host RNA polymerase were identified. One of them is an ECF-family s-factor that may be responsible for transcription of some viral genes. Another RNA polymerase-binding protein is a novel transcription inhibitor whose mechanism of action remains to be defined.