GENOMIC AND PROTEOMIC ANALYSIS OF PHI32, A NOVEL ESCHERICHIA COLI PHAGE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在大规模筛选可用于治疗牛乳腺炎的噬菌体的过程中，分离到一种感染大肠杆菌的新型噬菌体。对命名为phi 32的噬菌体的77，554 bp基因组进行了测序和注释，并通过电子显微镜和蛋白质组学对其病毒体进行了表征。鉴定了两种与宿主RNA聚合酶相互作用的phi 32编码蛋白。其中之一是ECF家族σ因子，其可能负责某些病毒基因的转录。另一种RNA聚合酶结合蛋白是一种新的转录抑制剂，其作用机制仍有待确定。 新型大肠杆菌噬菌体phiEco 32的基因组和蛋白质组分析 萨瓦利亚D，Westblade LF，Goel M，Florens L，肯普P，Akulenko N，Pavlova O，Padovan JC，Chait BT，Washburn MP，Ackermann HW，Mushegian A，Gabisonia T，Molineux I，Severinov K.分子生物学杂志2008年3月28日;377（3）：774-89。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A novel phage infecting Escherichia coli was isolated during a large-scale screen for phages that may be used for therapy of mastitis in cattle. The 77,554 bp genome of the phage, named phi32, was sequenced and annotated, and its virions were characterized by electron microscopy and proteomics. Two phi32-encoded proteins that interact with host RNA polymerase were identified. One of them is an ECF-family sigma-factor that may be responsible for transcription of some viral genes. Another RNA polymerase-binding protein is a novel transcription inhibitor whose mechanism of action remains to be defined. Genomic and proteomic analysis of phiEco32, a novel Escherichia coli bacteriophage. Savalia D, Westblade LF, Goel M, Florens L, Kemp P, Akulenko N, Pavlova O, Padovan JC, Chait BT, Washburn MP, Ackermann HW, Mushegian A, Gabisonia T, Molineux I, Severinov K. J Mol Biol. 2008 Mar 28;377(3):774-89.