STUDY OF HUMAN NUCLEAR PORE COMPLEX PROTEIN NUP358

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们用GFP标记的Nup 358的N-末端转染HeLa细胞，低温破坏细胞，并使用细胞粉末进行免疫亲和纯化。经过几次初步优化实验，我们已经证实了转染的N-末端与内源性全长Nup 358的相互作用，目前正在研究相互作用的伴侣。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We transfected HeLa cells with the GFP-tagged N-terminus of Nup358, cryogenically disrupted the cells, and used the cell powder for immunoaffinity purifications. After several initial optimization experiments, we have confirmed the interaction of the transfected N-terminus with the endogenous full length Nup358, and are currently studying the interacting partners.