STRUCTURE DETERMINATION OF SEC13 AND NUP96 COMPLEX

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 核孔复合体(NPC)是由至少30个不同蛋白质亚基组成的大分子结构。鼻咽癌最明显的作用是通过被动扩散或促进运输，成为大分子核质交换的唯一门户。NPC由三个不同的建筑结构组成：中央框架、细胞质细丝和核篮子。中央骨架被认为形成了连接细胞质和内核的通道。全国人大具有八重对称性。由于这种对称性，大多数核孔素的拷贝数应该是8或8的倍数。大多数核孔蛋白对称地分布在鼻咽癌两侧，少数分布在核面或胞浆面上。为了获得NPC建筑的完整图像，需要一个高分辨率的三维结构。然而，要在完整的结构上一次获得这样的地图是不可行的。Blobel实验室正在采取一种合作的方法来建立这样的模型。我已经将核孔复合体的两个组分Sec13和Nup96的一个片段囊化了。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The nuclear pore complex (NPC) is a large macromolecular structure composed of at least 30 different protein subunits. The most obvious roles of the NPC is being the sole gateway for nucleocytoplasmic exchange of macromolecules, either through passive diffusion or facilitated transported. The NPC consists of three distinct architectural structures: the central framework, the cytoplasmic filaments and the nuclear basket. The central framework is believed to form the channel that connects the cytoplasm and inner nucleus. The NPC contains an eight fold symmetry. Due do this symmetry, most nucleoporin should be present at a copy number of eight or a multiple of eight. Most of the nucleoporins are located symmetrically on both sides of the NPC, whereas only a few are positioned at either the nuclear or the cytoplasmic face. For a complete picture of the NPC architecture a high resolution three dimensional structure would be required. However, it is not feasible to obtain such a map on the complete structure at once. A collaborative approach taken by the Blobel lab to build such a model is underway. I have cocystalized two components of the nuclearpore complex, Sec13 and a fragment of Nup96.