STUDY OF HUMAN NUCLEAR PORE COMPLEX PROTEIN NUP358

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们用GFP标记的Nup358的N末端转染HeLa细胞，冷冻破坏细胞，并将细胞粉末用于免疫亲和纯化。经过几次初步的优化实验，我们已经证实了转染的N端与内源全长Nup358的相互作用，目前正在研究相互作用的伙伴。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We transfected HeLa cells with the GFP-tagged N-terminus of Nup358, cryogenically disrupted the cells, and used the cell powder for immunoaffinity purifications. After several initial optimization experiments, we have confirmed the interaction of the transfected N-terminus with the endogenous full length Nup358, and are currently studying the interacting partners.