INVESTIGATING THE FUNCTION OF NS4B OF BVDV

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 牛病毒性腹泻病毒，类似于丙型肝炎病毒，是黄病毒科的成员，在这一类别中，它是一种害虫病毒。马特·保尔森已经确定了BVDV中丙型肝炎病毒NS4B同源物的一个突变位置，这导致了感染后抵抗力的增加(细胞在感染后没有死亡)。我们的目标是首先确定正常蛋白和突变蛋白的细胞相互作用伙伴。我们已经比较了几种分离方法，并使用磁珠，到目前为止，我们已经确定了预期的相互作用伙伴之一。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Bovine Viral Diarrhea Virus, similarly to HCV, is a member of Flaviviridae, and within this category, it is a Pestivirus. Matt Paulson has identified a mutation site of the HCV NS4B homologue in BVDV which resulted in an increased resistance following the infection (the cells did not die after infection). We aim to first identify the cellular interacting partners of the normal and the mutant protein. We have compared several isolation methods and using magnetic beads so far we have identified one of the expected interacting partners.