PROTEIN MASS DETERMINATION WITH HIGH ACCURACY USING AN ESI-ION TRAP MS

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed a strategy that combines an instrumentation and software approach to measure the average molecular mass of proteins with accuracies of approximately 10ppm. The strategy consists of (1) producing an envelope of multiply charged protein ions avoiding space-charge effects, (2) calibrating the spectrum externally with an envelope of multiply charge ions from another protein (a calibrant), then (3) deconvoluting the ion envelope into a single mass peak centered at the average mass distribution of the protein being measured. The deconvolution software was written in our laboratory and has been presented at one of the conferences of the American Society for Mass Spectrometry and Allied Topics. A manuscript describing this approach is being prepared.