PROTEIN MASS DETERMINATION WITH HIGH ACCURACY USING AN ESI-ION TRAP MS

使用 ESI 离子阱 MS 高精度测定蛋白质质量

• 批准号: 7722243
• 负责人: JULIO C PADOVAN
• 金额: $ 0.22万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722243
• 关键词: Ally; American; Charge; Computer Retrieval of Information on Scientific Projects Database; Computer software; Funding; Grant; Institution; Ions; Laboratories; Manuscripts; Mass Spectrum Analysis; Measures; Proteins; Research; Research Personnel; Resources; Societies; Source; United States National Institutes of Health; Writing; instrumentation; molecular mass; protein distribution; symposium

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed a strategy that combines an instrumentation and software approach to measure the average molecular mass of proteins with accuracies of approximately 10ppm. The strategy consists of (1) producing an envelope of multiply charged protein ions avoiding space-charge effects, (2) calibrating the spectrum externally with an envelope of multiply charge ions from another protein (a calibrant), then (3) deconvoluting the ion envelope into a single mass peak centered at the average mass distribution of the protein being measured. The deconvolution software was written in our laboratory and has been presented at one of the conferences of the American Society for Mass Spectrometry and Allied Topics. A manuscript describing this approach is being prepared.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们开发了一种结合仪器和软件方法的策略，以测量蛋白质的平均分子量，精度约为10 ppm。该策略包括：（1）产生多电荷蛋白质离子的包络，避免空间电荷效应，（2）用另一种蛋白质（校准物）的多电荷离子包络在外部校准光谱，然后（3）将离子包络解卷积为以被测蛋白质的平均质量分布为中心的单个质量峰。去卷积软件是在我们的实验室编写的，并已在美国质谱和相关主题协会的一次会议上发表。目前正在编写一份说明这一办法的手稿。