RECONSTITUTION OF THE TYPE II [3H]ESTRADIOL BINDING SITE WITH RECOMBINANT

用重组体重建 II 型 [3H]雌二醇结合位点

• 批准号: 7721509
• 负责人: Kevin W Shoulars
• 金额: $ 0.44万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721509
• 关键词: Binding; Binding Sites; Computer Retrieval of Information on Scientific Projects Database; Estradiol; Funding; Grant; Histone H3; Histone H4; Histones; Institution; Ligand Binding; Methodology; Methods; Nuclear; Production; Property; Proteins; Rattus; Recombinants; Research; Research Design; Research Personnel; Resources; Site; Source; Techniques; Thymus Gland; United States National Institutes of Health; dimer; immunoreactivity; reconstitution

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Previously, we identified the rat uterine nuclear type II [3H]estradiol binding site as histone H4 and an unknown 35 kDa protein with histone H4 immunoreactivity. Studies using calf thymus histones indicated that the 35 kDa protein was likely a dimer of histone H3 and H4. Further study of the type II site required methodology for producing sufficient quantities of recombinant histones, which retained ligand- binding properties. A variety of production methods produce sufficient quantities of histone for binding analyses were evaluated prior to finding a successful technique.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 此前，我们鉴定了大鼠子宫核II型[~H]雌二醇结合部位为组蛋白H4，以及一个未知的具有组蛋白H4免疫反应性的35 kDa蛋白。对小牛胸腺组蛋白的研究表明，35 kDa的蛋白质很可能是组蛋白H3和H4的二聚体。对II型位点的进一步研究需要生产足够数量的重组组蛋白的方法学，这些重组蛋白保留了配体结合的特性。在找到一种成功的技术之前，对各种生产方法产生足够数量的组蛋白进行结合分析进行了评估。