SELENIUM DERIVATIZATION OF NUCLEIC ACIDS FOR X-RAY CRYSTALLOGRAPHY VIA MAD
通过 MAD 对核酸进行硒衍生化,用于 X 射线晶体学
基本信息
- 批准号:7726253
- 负责人:
- 金额:$ 2.41万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-09-18 至 2009-06-30
- 项目状态:已结题
- 来源:
- 关键词:Catalytic DNAChemicalsComplexComputer Retrieval of Information on Scientific Projects DatabaseCrystallographyDNAFundingGrantInstitutionLabelLinkMethodsNucleic AcidsOxygenPhaseProteinsRNARNA BindingRNA SequencesResearchResearch PersonnelResolutionResourcesRoentgen RaysSeleniumSourceStructureUnited States National Institutes of HealthX-Ray Crystallographynovelnovel strategiestool
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
X-ray crystallography is a powerful tool for structure determination of RNA structure, RNA-protein and DNA-protein complexes with high resolution. Derivatization with heavy atoms for phase determination, a long-standing problem in X-ray crystallography, however, has largely slowed down structural determination of nucleic acids with novel folds. One approach to facilitate the structure determination is to label nucleic acids with covalently linked heavy atoms, which enable phase and structure determination. We have developed a novel strategy to derivatize RNA and DNA by replacing oxygen with selenium, and this principle has been demonstrated by X-ray crystallography using MAD phasing method. We are derivatizing DNA and catalytic and binding RNA sequences with novel folds, via chemical and enzymatic synthesis, for X-ray crystallographic studies of nucleic acids and their protein complexes.
这个子项目是众多研究子项目之一
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('ZHEN HUANG', 18)}}的其他基金
BTEX Contaminated Groundwater Remediation by Bio-Electrochemical Systems
生物电化学系统修复受苯系物污染的地下水
- 批准号:
8781940 - 财政年份:2014
- 资助金额:
$ 2.41万 - 项目类别:
Selenium-Derivatized RNAs and DNAs for High-Throughput Protein/Nucleic Acid Cryst
用于高通量蛋白质/核酸晶体的硒衍生化 RNA 和 DNA
- 批准号:
8450750 - 财政年份:2012
- 资助金额:
$ 2.41万 - 项目类别:
Selenium-Derivatized RNAs and DNAs for High-Throughput Protein/Nucleic Acid Cryst
用于高通量蛋白质/核酸晶体的硒衍生化 RNA 和 DNA
- 批准号:
8238770 - 财政年份:2012
- 资助金额:
$ 2.41万 - 项目类别:
Selenium-Derivatized RNAs and DNAs for High-Throughput Protein/Nucleic Acid Cryst
用于高通量蛋白质/核酸晶体的硒衍生化 RNA 和 DNA
- 批准号:
8640953 - 财政年份:2012
- 资助金额:
$ 2.41万 - 项目类别:
Selenium-Derivatized RNAs and DNAs for High-Throughput Protein/Nucleic Acid Cryst
用于高通量蛋白质/核酸晶体的硒衍生化 RNA 和 DNA
- 批准号:
9044602 - 财政年份:2012
- 资助金额:
$ 2.41万 - 项目类别:
SELENIUM DERIVATIZATION OF NUCLEIC ACIDS FOR X-RAY CRYSTALLOGRAPHY VIA MAD
通过 MAD 对核酸进行硒衍生化,用于 X 射线晶体学
- 批准号:
7602320 - 财政年份:2007
- 资助金额:
$ 2.41万 - 项目类别:
SELENIUM DERIVATIZATION OF NUCLEIC ACIDS FOR X-RAY CRYSTALLOGRAPHY VIA MAD
通过 MAD 对核酸进行硒衍生化,用于 X 射线晶体学
- 批准号:
7358941 - 财政年份:2006
- 资助金额:
$ 2.41万 - 项目类别:
Se-Derivatization of Functional RNAs for Structure Study
用于结构研究的功能性 RNA 的 Se 衍生化
- 批准号:
6703228 - 财政年份:2004
- 资助金额:
$ 2.41万 - 项目类别:
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