CHARACTERIZATION OF THE PHYSICAL AND GENETIC INTERACTIONS OF CTF4

CTF4 的物理和遗传相互作用的表征

• 批准号: 7723758
• 负责人: Philip A. Hieter
• 金额: $ 0.73万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723758
• 关键词: Co-Immunoprecipitations; Computer Retrieval of Information on Scientific Projects Database; Evolution; Funding; Genes; Genetic; Genome Stability; Grant; Homologous Gene; Human; Institution; Malignant Neoplasms; Mass Spectrum Analysis; Mutate; Proteins; Research; Research Personnel; Resources; Saccharomyces cerevisiae; Saccharomycetales; Source; United States National Institutes of Health; Work; Yeasts; cancer therapy; member; yeast two hybrid system

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Work in the budding yeast Saccharomyces cerevisiae has uncovered a synthetic lethal genetic interaction network for genome stability genes, a few members of which have interactions with many other members of the network. One of these central genes is CTF4; it and many of its genetic interacting partners are conserved across evolution. Many of the human homologs of CTF4's genetic interactors have been previously shown to be mutated in human cancers. Probing the physical interactions of the Ctf4 protein would serve to fill out its interaction network, and may also reveal additional conserved targets for anticancer therapy. Interactors identified in a yeast two-hybrid screen will subsequently be confirmed by reciprocal two-hybrid interaction and co-immunoprecipitation/mass spectrometry.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在酿酒酵母的萌芽酵母菌糖果疗中的工作已经发现了基因组稳定基因的合成致命遗传相互作用网络，其中一些成员与网络的许多其他成员有相互作用。这些中心基因之一是CTF4；它及其许多遗传相互作用伙伴在进化过程中都是保守的。以前，CTF4遗传相互作用者的许多人类同源物已被证明在人类癌症中已突变。探测CTF4蛋白的物理相互作用将有助于填充其相互作用网络，并且还可能揭示了抗癌治疗的其他保守靶标。在酵母双杂化筛网中鉴定出的相互作用者将通过相互互动的两杂交相互作用和共免疫沉淀/质谱法证实。