CHARACTERIZATION OF ESSENTIAL PROTEINS INVOLVED IN CHROMOSOME SEGREGATION
CHARACTERIZATION OF ESSENTIAL PROTEINS INVOLVED IN CHROMOSOME SEGREGATION
批准号:
7957797
负责人:
Philip A. Hieter
金额:
$0.33万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-09-01 至 2010-08-31
关键词:
Affinity ChromatographyAnaphaseAneuploid CellsBiological ProcessBiologyCell divisionChromosomal InstabilityChromosome SegregationChromosomesComplexComputer Retrieval of Information on Scientific Projects DatabaseDNA DamageDNA RepairDefectEssential GenesExhibitsFundingFungal GenomeGenesGenetic ScreeningGenomicsGoalsGrantInstitutionKnowledgeLaboratoriesLeadMaintenanceMalignant NeoplasmsMammalsMetaphaseMethodsMitosisMutateNormal CellProcessProtein AnalysisProteinsResearchResearch PersonnelResourcesRoleSaccharomyces cerevisiaeSaccharomycetalesSister ChromatidSourceSystemTechniquesUnited States National Institutes of HealthYeastschromosome losscohesincohesiondaughter cellinterestmutantnovelprotein complextumor progression
中文摘要
这个子项目是许多研究子项目中利用
资源由NIH/NCRR资助的中心拨款提供。子项目和
调查员(PI)可能从NIH的另一个来源获得了主要资金,
并因此可以在其他清晰的条目中表示。列出的机构是
该中心不一定是调查人员的机构。
正常的细胞分裂需要所有的染色体被复制并平均分离成两个子细胞。在有丝分裂过程中,姐妹染色单体通过被称为粘附素的蛋白质复合体(通过被称为姐妹染色单体凝聚的过程)结合在一起,直到中期到后期的过渡。凝聚力缺陷可通过增加获得或丢失染色体的速率而导致非整倍体细胞(称为染色体不稳定(CIN))。在哺乳动物系统中,CIN可能促进癌症进展,并在各种癌症中表现出来,但其发生机制尚不清楚。发芽酵母酿酒酵母可用于鉴定哺乳动物中与维持基因组完整性和保守性有关的基因。Hieter实验室最近对ts突变资源进行了遗传筛选,发现了三个新的必需基因,这些基因在突变时会导致CIN、凝聚力缺陷和对DNA损伤剂的敏感性。为了充分了解相应蛋白质的生物学功能,该项目的一个主要目标是确定相互作用的蛋白质,这些蛋白质可能产生关于这些蛋白质参与哪个特定生物过程的信息。使用的主要技术将是串联亲和纯化(TAP),以分离目的蛋白作为完整复合体的一部分,并确定相互作用的伙伴。TAP方法已被证明可用于分离具有足够纯度的生物活性络合物,用于质谱分析和蛋白质鉴定。TAP获得的结果不仅将扩大我们对酵母中蛋白质相互作用网络的了解,还将为进一步表征这些新蛋白质在DNA修复和姐妹染色单体凝聚力建立和/或维持中的作用提供一个起点。
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Normal cell division requires that all the chromosomes are duplicated and separated equally into the two daughter cells. During mitosis, sister chromatids are held together by protein complexes known as cohesins (by a process known as sister chromatid cohesion), until the metaphase to anaphase transition. Defective cohesion can lead to aneuploid cells by an increased rate of gain or loss of chromosomes (known as chromosome instability (CIN)). In mammalian systems, CIN may contribute to cancer progression and is exhibited by a variety of cancers, yet the mechanism by which it occurs is poorly understood. The budding yeast Saccharomyces cerevisiae has been useful for identifying genes that are involved in maintenance of genomic integrity and conserved in mammals. A recent genetic screen of a Ts mutant resource in the Hieter laboratory yielded three novel essential genes that lead to CIN, cohesion defects and sensitivity to DNA damaging agents when mutated. In order to fully understand the biological function of the corresponding proteins, a primary goal of this project is to identify interacting proteins that may yield information about which specific biological process these proteins are involved in. The primary technique used will be tandem affinity purification (TAP) in order to isolate the proteins of interest as part of intact complexes and to identify interacting partners. The TAP method has been shown to be useful for isolating biologically active complexes of sufficient purity for mass spectrometric analysis and protein identification. The results obtained from TAP will not only expand our knowledge of protein interaction networks in yeast but will also provide a starting point to further characterize the role of these novel proteins in DNA repair and sister chromatid cohesion establishment and/or maintenance.
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Functional Determinants of Chromosome Segregation
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批准号:8594232
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项目类别:
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资助金额:$21.74万
-
财政年份:2012
-
负责人:Philip A. Hieter
-
依托单位:
Functional Determinants of Chromosome Segregation
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批准号:8784057
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项目类别:
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资助金额:$22.41万
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财政年份:2012
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负责人:Philip A. Hieter
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依托单位:
Functional Determinants of Chromosome Segregation
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批准号:8434750
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项目类别:
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资助金额:$21.07万
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财政年份:2012
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负责人:Philip A. Hieter
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依托单位:
Functional Determinants of Chromosome Segregation
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批准号:8235734
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项目类别:
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资助金额:$22.41万
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财政年份:2012
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负责人:Philip A. Hieter
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依托单位:
CHARACTERIZATION OF THE PHYSICAL AND GENETIC INTERACTIONS OF CTF4
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批准号:7723758
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项目类别:
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资助金额:$0.73万
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财政年份:2008
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负责人:Philip A. Hieter
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依托单位:
CHARACTERIZATION OF ESSENTIAL PROTEINS INVOLVED IN CHROMOSOME SEGREGATION
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批准号:7723753
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项目类别:
-
资助金额:$0.67万
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财政年份:2008
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负责人:Philip A. Hieter
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依托单位:
IDENTIFICATION OF POTENTIAL NDC10 INTERACTING PROTEINS BY TWO-HYBRID
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批准号:7602236
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项目类别:
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资助金额:$1.04万
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财政年份:2007
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负责人:Philip A. Hieter
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依托单位:
STRUCUTRAL PREDICTION OF THE YEAST KINETOCHORE PROTEIN NDC10
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批准号:7420746
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项目类别:
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资助金额:$0.83万
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财政年份:2006
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负责人:Philip A. Hieter
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依托单位:
IDENTIFICATION OF PROTEINS INTERACTING WITH MMS22P, RTT101P AND RTT107P
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批准号:7182337
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项目类别:
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资助金额:$0.66万
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财政年份:2005
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负责人:Philip A. Hieter
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依托单位:
FUNCTIONAL DETERMINANTS OF CHROMOSOME SEGREGATION IN YEAST
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批准号:7049162
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项目类别:
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资助金额:$18.84万
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财政年份:2005
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负责人:Philip A. Hieter
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依托单位:
GENOMIC Y2H SCREEN OF CTF18, CTF8 AND DCC1
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批准号:7182395
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项目类别:
-
资助金额:$0.66万
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财政年份:2005
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负责人:Philip A. Hieter
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依托单位:
IDENTIFICATION OF APC/C SUBSTRATES
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批准号:6979545
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项目类别:
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资助金额:$2.27万
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财政年份:2004
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负责人:Philip A. Hieter
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依托单位:
RAD61P ROLE IN CHROMOSOME SEGREGATION
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批准号:6979652
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项目类别:
-
资助金额:$0.34万
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财政年份:2004
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负责人:Philip A. Hieter
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依托单位:
IDENTIFICATION OF THE RFC (CTF8) COMPLEX
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批准号:6979542
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项目类别:
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资助金额:$0.41万
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财政年份:2004
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负责人:Philip A. Hieter
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依托单位:
IDENTIFICATION OF POTENTIAL NDC10 INTERACTING PROTEINS
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批准号:6979562
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项目类别:
-
资助金额:$0.34万
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财政年份:2004
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负责人:Philip A. Hieter
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依托单位:
LOCALIZATION OF SELECTED BUDDING YEAST APC/C SUBUNITS
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批准号:6979568
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项目类别:
-
资助金额:$1.02万
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财政年份:2004
-
负责人:Philip A. Hieter
-
依托单位:
IDENTIFICATION OF APC/C SUBSTRATES
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批准号:6979629
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项目类别:
-
资助金额:$0.41万
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财政年份:2004
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负责人:Philip A. Hieter
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依托单位:
FUNCTIONAL DETERMINANTS OF CHROMOSOME SEGREGATION
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批准号:6101792
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项目类别:
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资助金额:$32.18万
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财政年份:1999
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负责人:Philip A. Hieter
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依托单位:
FUNCTIONAL DETERMINANTS OF CHROMOSOME SEGREGATION
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批准号:6268917
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项目类别:
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资助金额:$30.95万
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财政年份:1998
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负责人:Philip A. Hieter
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依托单位:
APPROACHES IN YEAST TO STUDY OF ANEUPLOIDY
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批准号:6108514
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项目类别:
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资助金额:$0.0万
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财政年份:1998
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负责人:Philip A. Hieter
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依托单位:
国内基金
海外基金
RIF1蛋白在处理超细后期桥(ultrafine anaphase bridge)和保障基因组稳定的作用
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批准号:
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项目类别:省市级项目
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资助金额:10.0万元
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批准年份:2019
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负责人:陈英伟
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依托单位: