STRUCUTRAL PREDICTION OF THE YEAST KINETOCHORE PROTEIN NDC10

酵母着丝粒蛋白 NDC10 的结构预测

• 批准号: 7420746
• 负责人: Philip A. Hieter
• 金额: $ 0.83万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420746
• 关键词: arginine; cell cycle; centromere; chromosome movement; emotions; gene mutation; genetics; genome; lysine; point mutation; protein structure; proteins; role; social integration; yeasts

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ndc10p and more than sixty other proteins function at the budding yeast kinetochore in chromosome segregation to ensure proper partitioning of genetic material. To avoid errors in segregation this process is highly regulated and intimately coordinated with other mitotic events through the action of cell cycle checkpoints. Post-translational modification of kinetochore proteins and other cell cycle machinery is one mechanism used by the cell to control these events. In a genome wide two-hybrid screen, multiple components of the sumoylation machinery (Smt3p, Nfi1p, and Ubc9p) were identified as two-hybrid interactors of Ndc10p. To identify lysine residues participating in sumoylation, we generated lysine to arginine point mutations in Ndc10p. Three key lysine residues were identified that affect Ndc10p sumoylation levels when mutated. When combined these mutations cause increased rates of chromosome segregation errors and perturbs the localization pattern of Ndc10p indicating a critical role for Ndc10p sumoylation in the fidelity of the mitotic process.To understand the effect these mutations have we propose to use structural predictions of protein structure on to which these mutations can be mapped.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。 NDC10P和六十多个蛋白质在染色体隔离的芽酵母动物学中起作用，以确保对遗传物质的适当分配。为了避免隔离错误，此过程受到高度调节，并通过细胞周期检查点的作用与其他有丝分裂事件密切协调。动力学蛋白和其他细胞周期机制的翻译后修饰是细胞控制这些事件的一种机制。在基因组宽的两杂种屏幕中，Sumoylation机械的多个组件（SMT3P，NFI1P和UBC9P）被鉴定为NDC10P的两个杂交相互作用者。为了鉴定参与Sumoylation的赖氨酸残基，我们在NDC10P中生成了赖氨酸至精氨酸点突变。突变时，鉴定出三个关键的赖氨酸残基会影响NDC10P Sumoylation水平。当组合结合后，这些突变导致染色体隔离误差的速率增加，并使NDC10P的定位模式变为NDC10P Sumoylation在有丝分裂过程的保真度中起着至关重要的作用。要了解这些突变的效果，我们建议使用这些突变的蛋白质结构来映射这些突变的结构结构。