TOTAL OXIDATIVE POST TRANSLATIONAL MODIFICATION MAP OF H-RAS

H-RAS 的总氧化翻译后修饰图谱

• 批准号: 7723028
• 负责人: PETER B. O'CONNOR
• 金额: $ 0.26万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723028
• 关键词: Analytical Chemistry; Computer Retrieval of Information on Scientific Projects Database; Condition; Funding; Goals; Grant; In Vitro; Institution; Maps; Methods; Modification; Oxidative Stress; Paper; Peroxonitrite; Post-Translational Protein Processing; Proteomics; Publishing; Research; Research Personnel; Resources; SHFM1 gene; Sampling; Source; Techniques; United States National Institutes of Health; Work; in vivo Model

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project aimed to use the unique capabilities of our recently developed ESI-qQq-FTMS to fully characterize the post-translational modifications of H-RAS that occur during oxidative stress conditions. The project used a combination of techniques including top-down and bottom-up proteomics methods, selective accumulation, Q2-CAD, and ECD. The samples involved are commercially purchased H-RAS, both native state and oxidatively modified with peroxynitrite. This was done in-vitro initially, but the goal is to extend this to in-vivo models once the in-vitro modifications are characterized. A paper was published in Analytical Chemistry on this work.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目旨在使用我们最近开发的ESI-qQq-FTMS的独特功能来充分表征氧化应激条件下发生的H-RAS的翻译后修饰。 该项目使用了一系列技术，包括自上而下和自下而上的蛋白质组学方法，选择性积累，Q2-CAD和ECD。 所涉及的样品是市售的H-RAS，天然状态和用过氧亚硝酸盐氧化改性的。 这最初是在体外完成的，但目标是一旦体外修饰被表征，就将其扩展到体内模型。 有关这项工作的论文发表在《分析化学》上。