CHARACTERIZATION OF SATB1 MODIFICATIONS FOLLOWING IONIZATION RADIATION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 引言和基本原理我的实验室的一个主要目标是了解大量基因以细胞类型或阶段特异性方式或响应外部信号而被表达或抑制的基本机制。 在这种情况下，我们确定了一个核结构蛋白，SATB 1，作为一个全球性的基因调节器。SATB 1是一种磷酸化蛋白。 我们希望测试一个假设，即SATB 1蛋白在辐照细胞中的翻译后修饰发生变化。 我们希望完成正在进行的合作实验，以确定是否有任何变化的磷酸化状态以及其他翻译后修饰，SATB 1在胸腺细胞之前和之后细胞暴露于电离辐射。 该项目的目标是为SATB 1蛋白的每种翻译后修饰分配特定的功能，类似于“组蛋白密码”的概念。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction and Rationale A major goal of my laboratory has been to understand the fundamental mechanisms by which a large body of genes is directed to be either expressed or repressed in a cell-type or stage-specific manner or in response to signals from outside. In this context, we identified a nuclear architectural protein, SATB1, which functions as a global gene regulator. SATB1 is a phosphorylated protein. We wish to test a hypothesis that the SATB1 protein undergoes changes in post-translational modification in irradiated cells. We wish to complete the ongoing collaborative experiment, to determine if there is any change in the phosphorylation status as well as other post-translational modifications, of SATB1 in thymocytes before and after cells are exposed to ionizing radiation. The goal of the project is to assign specific functions to each type of post-translational modification of the SATB1 protein, similar to the concept of the 'histone code.