CHARACTERIZATION OF SATB1 MODIFICATIONS FOLLOWING IONIZATION RADIATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 前言和基本原理我的实验室的一个主要目标是了解大量基因被指示以细胞类型或阶段特有的方式或对外界信号做出反应而表达或抑制的基本机制。在此背景下，我们发现了一种核结构蛋白SATB1，它具有全球基因调控功能。SATB1是一种磷酸化的蛋白质。我们希望检验一种假设，即SATB1蛋白在受辐射的细胞中经历翻译后修饰的变化。我们希望完成正在进行的合作实验，以确定在细胞暴露于电离辐射前后，胸腺细胞中SATB1的磷酸化状态以及其他翻译后修饰是否有任何变化。该项目的目标是为SATB1蛋白的每一种翻译后修饰赋予特定的功能，类似于组蛋白密码的概念。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction and Rationale A major goal of my laboratory has been to understand the fundamental mechanisms by which a large body of genes is directed to be either expressed or repressed in a cell-type or stage-specific manner or in response to signals from outside. In this context, we identified a nuclear architectural protein, SATB1, which functions as a global gene regulator. SATB1 is a phosphorylated protein. We wish to test a hypothesis that the SATB1 protein undergoes changes in post-translational modification in irradiated cells. We wish to complete the ongoing collaborative experiment, to determine if there is any change in the phosphorylation status as well as other post-translational modifications, of SATB1 in thymocytes before and after cells are exposed to ionizing radiation. The goal of the project is to assign specific functions to each type of post-translational modification of the SATB1 protein, similar to the concept of the 'histone code.