CHARACTERIZATION OF SATB1 MODIFICATIONS FOLLOWING IONIZATION RADIATION

电离辐射后 SATB1 修饰的表征

• 批准号: 8170703
• 负责人: Terumi Kohwi-Shigematsu
• 金额: $ 3.21万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170703
• 关键词: Adult; Affinity; Binding Proteins; Computer Retrieval of Information on Scientific Projects Database; DNA; Funding; Genes; Goals; Grant; Histone Code; Institution; Laboratories; Modification; Nuclear; Post-Translational Protein Processing; Proteins; Radiation; Regulator Genes; Research; Research Personnel; Resources; Signal Transduction; Source; Staging; Stem cells; Testing; United States National Institutes of Health; adult stem cell; base; cell type; embryonic stem cell; ionization; irradiation; response; thymocyte

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction and Rationale A major goal of my laboratory has been to understand the fundamental mechanisms by which a large body of genes is directed to be either expressed or repressed in a cell-type or stage-specific manner or in response to signals from outside. In this context, we identified a nuclear architectural protein, SATB1, which functions as a global gene regulator, expressed in embryonic stem (ES) cells and adult progenitor cells, such as thymocytes. Using a DNA affinity column containing BUR (base unpairing region) specifically recognized by SATB1, we wish to identify SATB1 associated proteins as well as new BUR-binding proteins in stem cells and adult progenitor cells. Furthermore, we wish to test a hypothesis that the SATB1 protein undergoes changes in post-translational modification during differentiation or upon irradiation. The goal of the project is to assign specific functions to each type of post-translational modification of the SATB1 protein, similar to the concept of the 'histone code'.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 引言和基本原理我的实验室的一个主要目标是了解大量基因以细胞类型或阶段特异性方式或响应外部信号而被表达或抑制的基本机制。 在这种情况下，我们确定了一个核结构蛋白，SATB 1，作为一个全球性的基因调节器，表达在胚胎干细胞（ES）和成人祖细胞，如胸腺细胞。 我们希望使用含有SATB 1特异性识别的BUR（碱基非配对区）的DNA亲和柱，鉴定SATB 1相关蛋白以及干细胞和成人祖细胞中新的BIR结合蛋白。 此外，我们希望测试一个假设，即SATB 1蛋白在分化过程中或照射后的翻译后修饰发生变化。该项目的目标是为SATB 1蛋白的每种翻译后修饰分配特定的功能，类似于“组蛋白密码”的概念。