CHARACTERIZATION OF SATB1 MODIFICATIONS FOLLOWING IONIZATION RADIATION

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 引言和基本原理我的实验室的一个主要目标是了解大量基因以细胞类型或阶段特异性方式或响应外部信号而被表达或抑制的基本机制。 在这种情况下，我们确定了一个核结构蛋白，SATB 1，作为一个全球性的基因调节器，表达在胚胎干细胞（ES）和成人祖细胞，如胸腺细胞。 使用含有SATB 1特异性识别的BUR（碱基不配对区）的DNA亲和柱，我们希望在干细胞和成体祖细胞中鉴定SATB 1相关蛋白以及新的BUR结合蛋白。 此外，我们希望测试一个假设，即SATB 1蛋白在分化过程中或照射后的翻译后修饰发生变化。该项目的目标是为SATB 1蛋白的每种翻译后修饰分配特定的功能，类似于“组蛋白密码”的概念。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction and Rationale A major goal of my laboratory has been to understand the fundamental mechanisms by which a large body of genes is directed to be either expressed or repressed in a cell-type or stage-specific manner or in response to signals from outside. In this context, we identified a nuclear architectural protein, SATB1, which functions as a global gene regulator, expressed in embryonic stem (ES) cells and adult progenitor cells, such as thymocytes. Using a DNA affinity column containing BUR (base unpairing region) specifically recognized by SATB1, we wish to identify SATB1 associated proteins as well as new BUR-binding proteins in stem cells and adult progenitor cells. Furthermore, we wish to test a hypothesis that the SATB1 protein undergoes changes in post-translational modification during differentiation or upon irradiation. The goal of the project is to assign specific functions to each type of post-translational modification of the SATB1 protein, similar to the concept of the 'histone code'.