CHARACTERIZATION OF SATB1 MODIFICATIONS FOLLOWING IONIZATION RADIATION

电离辐射后 SATB1 修饰的表征

• 批准号: 8170703
• 负责人: Terumi Kohwi-Shigematsu
• 金额: $ 3.21万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170703
• 关键词: Adult; Affinity; Binding Proteins; Computer Retrieval of Information on Scientific Projects Database; DNA; Funding; Genes; Goals; Grant; Histone Code; Institution; Laboratories; Modification; Nuclear; Post-Translational Protein Processing; Proteins; Radiation; Regulator Genes; Research; Research Personnel; Resources; Signal Transduction; Source; Staging; Stem cells; Testing; United States National Institutes of Health; adult stem cell; base; cell type; embryonic stem cell; ionization; irradiation; response; thymocyte

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction and Rationale A major goal of my laboratory has been to understand the fundamental mechanisms by which a large body of genes is directed to be either expressed or repressed in a cell-type or stage-specific manner or in response to signals from outside. In this context, we identified a nuclear architectural protein, SATB1, which functions as a global gene regulator, expressed in embryonic stem (ES) cells and adult progenitor cells, such as thymocytes. Using a DNA affinity column containing BUR (base unpairing region) specifically recognized by SATB1, we wish to identify SATB1 associated proteins as well as new BUR-binding proteins in stem cells and adult progenitor cells. Furthermore, we wish to test a hypothesis that the SATB1 protein undergoes changes in post-translational modification during differentiation or upon irradiation. The goal of the project is to assign specific functions to each type of post-translational modification of the SATB1 protein, similar to the concept of the 'histone code'.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 引言和理由是我实验室的一个主要目标是了解大量基因被指示以细胞类型或特定于阶段的方式表达或抑制大量基因的基本机制，或者是针对外部信号的响应。 在这种情况下，我们确定了一种核结构蛋白SATB1，该蛋白在胚胎干细胞（ES）细胞和成年祖细胞（例如胸腺细胞）中表达的全球基因调节剂。 我们希望使用SATB1专门识别的BUR（基础不富裕区域）的DNA亲和力柱，我们希望鉴定SATB1相关的蛋白质以及干细胞和成人祖细胞中的新的bur结合蛋白。 此外，我们希望检验一个假设，即SATB1蛋白在分化期间或辐照过程中经历了翻译后修饰的变化。该项目的目的是为SATB1蛋白的每种类型的翻译后修饰分配特定功能，类似于“组蛋白代码”的概念。