CHARACTERIZATION OF SATB1 MODIFICATIONS FOLLOWING IONIZATION RADIATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 前言和基本原理我的实验室的一个主要目标是了解大量基因被指示以细胞类型或阶段特有的方式或对外界信号做出反应而表达或抑制的基本机制。在此背景下，我们确定了一种核结构蛋白SATB1，它具有全球基因调节功能，在胚胎干细胞(ES)和成体祖细胞(如胸腺细胞)中表达。利用含有SATB1特异性识别的碱基非配对区(BUR)的DNA亲和层析柱，我们希望在干细胞和成体祖细胞中鉴定SATB1相关蛋白以及新的BUR结合蛋白。此外，我们希望检验一个假设，即SATB1蛋白在分化过程中或辐射后的翻译后修饰发生变化。该项目的目标是为SATB1蛋白的每种翻译后修饰赋予特定的功能，类似于“组蛋白密码”的概念。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction and Rationale A major goal of my laboratory has been to understand the fundamental mechanisms by which a large body of genes is directed to be either expressed or repressed in a cell-type or stage-specific manner or in response to signals from outside. In this context, we identified a nuclear architectural protein, SATB1, which functions as a global gene regulator, expressed in embryonic stem (ES) cells and adult progenitor cells, such as thymocytes. Using a DNA affinity column containing BUR (base unpairing region) specifically recognized by SATB1, we wish to identify SATB1 associated proteins as well as new BUR-binding proteins in stem cells and adult progenitor cells. Furthermore, we wish to test a hypothesis that the SATB1 protein undergoes changes in post-translational modification during differentiation or upon irradiation. The goal of the project is to assign specific functions to each type of post-translational modification of the SATB1 protein, similar to the concept of the 'histone code'.