MOUSE METABOLIC PHENOTYPING CENTER

小鼠代谢表型中心

• 批准号: 7724108
• 负责人: Craig R Malloy
• 金额: $ 5.23万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724108
• 关键词: Animal Model; Animals; Basic Science; Biological Assay; Cations; Citric Acid Cycle; Computer Retrieval of Information on Scientific Projects Database; Computers; Data; Detection; Disease; Fatty acid glycerol esters; Funding; Gluconeogenesis; Glucose; Glycerol; Grant; Hepatic; Institution; Internet; Life; Liver; Measures; Metabolic; Metabolism; Methods; Modeling; Mus; NMR Spectroscopy; Non-Insulin-Dependent Diabetes Mellitus; Phenotype; Physicians; Protocols documentation; Pyruvate; Pyruvates; Recycling; Research; Research Personnel; Resources; Scientist; Series; Site; Source; Standards of Weights and Measures; Techniques; Testing; Tracer; United States National Institutes of Health; design; glucose production; improved; in vivo; oxidation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The aim of this project is to establish standard mouse phenotyping techniques to quantitatively evaluate liver gluconeogenesis, pyruvate recycling, the contribution of glycerol and Krebs cycle intermediates to glucose production, total glucose turnover, and citric acid cycle flux in live animals using a combination of 13C and 2H tracers and NMR spectroscopy. This standard metabolic assay provides the most comprehensive existing analysis of hepatic intermediary metabolism. It will be made available to on-campus and off-campus investigators having animal models of NIDDM or related disorders. In addition to the standard metabolic profile described above, a series of other phenotype specific metabolic techniques including a quantitative measure of substrate oxidation, measures of intracellular cations, and citric acid cycle flux using 13C enriched substrates will also be made available for selected animal models. A unique feature of this phenotyping center is that input will come from chemists, physicists, physiologists, computer scientists, physicians, and geneticists. All are recognized experts in 13C isotopomer analysis. A 600 MHz NMR spectrometer will be dedicated to this project. All mice selected for phenotyping will be subjected to a standard protocol performed by high-level technicians; the data will be analyzed and interpreted by NMR spectroscopists and biochemists and made available through an interactive web site designed to allow the external investigators to test a fit of their isotopomer data to alternative metabolic models. A basic research core will focus on improving sensitivity through indirect detection methods and develop methods to measure fat oxidation in vivo. Finally, an administrative core will coordinate the activities of the three research cores and interface discussions between external investigators and center scientists.

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