GENERALIZED EXTENSIONS OF CORRELATION SPECTROSCOPY

相关光谱学的广义扩展

• 批准号: 7724047
• 负责人: PAUL WISEMAN
• 金额: $ 0.35万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724047
• 关键词: Adhesions; Binding; Cells; Computer Retrieval of Information on Scientific Projects Database; Diffusion; Fluorescence; Fluorescence Resonance Energy Transfer; Focal Adhesions; Funding; Grant; Image; Institution; Life; Lipids; Maps; Measurement; Membrane; Methods; Microscope; Proteins; Research; Research Personnel; Resources; Series; Source; Spectrum Analysis; System; Time; United States National Institutes of Health; cell type; detector; macromolecule; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The project involves extending the paradigm of spatio-temporal fluorescence correlation to include the calculation of pairwise correlation functions. Essentially this will allow measurement of pairwise correlation functions from two spatially separated pixels in an image time series. The analysis of pairwise correlation functions should prove useful for mapping membrane domains by detecting that barriers exist in the cell that hinder free diffusion. An appropriate membrane system (protein or lipid) and cell type will be selected for trial experiments. We will also explore other extensions of image correlation spectroscopy, specifically developing line kICS (reciprocal or k-space method) to separate binding and unbinding from transport for measurements of adhesion related macromolecules within the context of focal adhesions in living cells. Finally we will explore the measurement of FRET via a microscope equipped with APD detectors.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目涉及扩展时空荧光相关的范例，包括成对相关函数的计算。本质上，这将允许从图像时间序列中的两个空间分离的像素测量成对相关函数。成对相关函数的分析应证明是有用的映射膜域检测障碍存在于细胞中，阻碍自由扩散。将选择适当的膜系统（蛋白质或脂质）和细胞类型进行试验实验。 我们还将探索图像相关光谱的其他扩展，特别是开发线kICS（倒数或k空间方法），以分离结合和解结合的运输粘附相关的大分子的测量范围内的活细胞中的粘着斑。 最后，我们将探讨通过配备APD探测器的显微镜测量FRET。