SYNTH & BIOL ACT OF TRANS RETINOL METABOLITE PRODUCE THRU NEW RETINOL METAB PAT

合成器

• 批准号: 7721607
• 负责人: Hector DeLuca
• 金额: $ 0.06万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721607
• 关键词: All-Trans-Retinol; Biological; COSY; Computer Retrieval of Information on Scientific Projects Database; Conceptus; Female; Fetal Resorption; Funding; Grant; Growth and Development function; Institution; Isomerism; Physiological Processes; Pregnancy; Rattus; Reproduction; Research; Research Personnel; Resources; Source; Spectrum Analysis; Tretinoin; United States National Institutes of Health; Vision; Vitamin A; day; dietary supplements; feeding; metabolic abnormality assessment; novel; nuclear Overhauser enhancement; prevent

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Retinol and its metabolites participate in many physiological processes, such as growth, development, reproduction, cellular differentiation, and vision. Rats fed with retinol-free and all-trans-retinoic acid (atRA)-supplemented diet fail to reproduce. It has been shown that retinol is required no later then day 10 of gestation in order to prevent fetal resorption in the vitamin A deficient, atRA-supplemented (VAD-RAS) female rats. Metabolic studies on all trans retinol in day 10 conceptuses of vitamin A deficient, retinoic acid supplemented rats led to the isolation and identification of a novel metabolite, the isomers of 2-(hydroxymethyl)-3-methyl-5-(2'-oxopropyl)-2,5-dihydrothiophene. In continuation of our biological studies of this metabolite, we synthesised the cis and trans isomers of the metabolite. Configurations of these final isomers as well as their precursors are to be determined by 1H, 1H COSY 2D spectra and 1H NOE difference spectroscopy to compare the isomers with the naturally occurring ones.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 视黄醇及其代谢产物参与多种生理过程，如生长、发育、生殖、细胞分化和视觉等。用不含视黄醇和全反式维甲酸(AtRA)的饲料喂养的大鼠不能繁殖。为了防止维生素A缺乏、全反式维甲酸(VAD-RAS)补充的雌性大鼠的胎儿吸收，研究表明，不迟于妊娠第10天需要视黄醇。维生素A缺乏补充维甲酸大鼠胚胎第10天的全反式视黄醇代谢研究导致分离和鉴定了一种新的代谢物2-(hydroxymethyl)-3-methyl-5-(2‘-oxopropyl)-2，5-dihydrothiophene.的异构体在继续我们对这种代谢物的生物学研究中，我们合成了代谢物的顺式和反式异构体。这些最终异构体及其前体的构型将通过1H，1H COSY 2D谱和1H NOE差谱来确定，以将异构体与自然发生的异构体进行比较。