MOUSE BRAIN ATLAS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们之前对染色活跃的小鼠大脑所做的工作使我们能够看到精确到20微米的细胞结构。但是，在这种分辨率水平上，我们对对比度来源的理解是相当重要的。这个项目的目标是利用9T磁共振磁铁更全面地了解活跃染色的小鼠大脑中细胞结构的对比来源。 1.我们将获得40微米的小鼠大脑的定量T1和T2图，并伴随着更高分辨率的自旋回波(T1加权)图像，以定义细胞结构。 2.我们将对相同的大脑进行组织切片，并对得到的数据集进行配准，以产生尼塞尔和甲酚紫的3D光学切片数据。 3.MR和光学切片将被登记为一卷。 4.结果数据将被用来定义活跃染色的大脑最关键区域的具体对比来源。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our previous work with actively stained mouse brains has allowed us to see the cytoarchitecture down to 20 microns. But, our understanding of the sources of contrast at this level of resolution is quite primative. The goal of this project is to understand more fully the sources of contrast of the cytoarchitecture in the actively stained mouse brain using the 9T MR magnet. 1. We will acquire quantitative T1 and T2 maps of the mouse brain at 40 microns with accompanying higher resolution spin echo (T1-weighted) images for definition of the cytoarchitecture. 2. We will histologically section the same brains and register the resulting datasets to produce 3D optical sectioned data with Nissl and Cresyl violet. 3. MR and optical sections will be registered into single volumes. 4. The resulting data will be used to define the specific sources of contrast in the most critical regions of the actively stained brain.