MOUSE BRAIN ATLAS

小鼠大脑图谱

• 批准号: 8363148
• 负责人: George ALLAN JOHNSON
• 金额: $ 1.84万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363148
• 关键词: Brain; Data; Data Set; Funding; Goals; Grant; Histologic; Image; Maps; Microscopy; Mus; National Center for Research Resources; Optics; Principal Investigator; Research; Research Infrastructure; Resolution; Resources; Source; Staining method; Stains; United States National Institutes of Health; Weight; Work; cost; cresyl violet

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our previous work with actively stained mouse brains has allowed us to see the cytoarchitecture down to 20 microns. But, our understanding of the sources of contrast at this level of resolution is quite primative. The goal of this project is to understand more fully the sources of contrast of the cytoarchitecture in the actively stained mouse brain using the 9T MR magnet. 1. We will acquire quantitative T1 and T2 maps of the mouse brain at 40 microns with accompanying higher resolution spin echo (T1-weighted) images for definition of the cytoarchitecture. 2. We will histologically section the same brains and register the resulting datasets to produce 3D optical sectioned data with Nissl and Cresyl violet. 3. MR and optical sections will be registered into single volumes. 4. The resulting data will be used to define the specific sources of contrast in the most critical regions of the actively stained brain.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们之前对活跃染色的小鼠大脑的工作使我们能够看到20微米的细胞结构。但是，我们对这个分辨率水平上的对比度来源的理解是相当原始的。本项目的目标是更全面地了解使用9 T MR磁体的活性染色小鼠脑中的细胞结构的对比度的来源。 1.我们将在40微米处获得小鼠大脑的定量T1和T2图，并伴随更高分辨率的自旋回波（T1加权）图像，用于定义细胞结构。 2.我们将对相同的大脑进行组织切片，并将所得数据集配准，以使用尼氏和甲酚紫产生3D光学切片数据。 3. MR和光学切片将配准到单个卷中。 4.所得数据将用于定义活性染色大脑最关键区域中的特定对比度来源。