MOUSE BRAIN ATLAS

小鼠大脑图谱

• 批准号: 8363148
• 负责人: George ALLAN JOHNSON
• 金额: $ 1.84万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363148
• 关键词: Brain; Data; Data Set; Funding; Goals; Grant; Histologic; Image; Maps; Microscopy; Mus; National Center for Research Resources; Optics; Principal Investigator; Research; Research Infrastructure; Resolution; Resources; Source; Staining method; Stains; United States National Institutes of Health; Weight; Work; cost; cresyl violet

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our previous work with actively stained mouse brains has allowed us to see the cytoarchitecture down to 20 microns. But, our understanding of the sources of contrast at this level of resolution is quite primative. The goal of this project is to understand more fully the sources of contrast of the cytoarchitecture in the actively stained mouse brain using the 9T MR magnet. 1. We will acquire quantitative T1 and T2 maps of the mouse brain at 40 microns with accompanying higher resolution spin echo (T1-weighted) images for definition of the cytoarchitecture. 2. We will histologically section the same brains and register the resulting datasets to produce 3D optical sectioned data with Nissl and Cresyl violet. 3. MR and optical sections will be registered into single volumes. 4. The resulting data will be used to define the specific sources of contrast in the most critical regions of the actively stained brain.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 我们以前使用的染色小鼠大脑的工作使我们能够看到细胞结构降低到20微米。但是，我们对在这种分辨率水平上对比来源的理解是相当原始的。该项目的目的是使用9T MR磁体更充分地了解主动染色的小鼠大脑中细胞结构的对比来源。 1。我们将以40微米的形式获取小鼠脑的定量T1和T2图，并伴随更高分辨率的自旋回声（T1加权）图像，以定义细胞结构。 2。我们将在组织学上分割相同的大脑，并注册所得数据集，以使用NISSL和Cresyl Violet产生3D光学截面数据。 3。MR和光学部分将注册为单卷。 4。所得数据将用于定义主动染色大脑最关键区域的特定对比来源。