COMPARATIVE TEM RECONSTRUCTION OF NEMATODE FEEDING STRUCTURES

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 拟议的研究解决了植物寄生线虫在系统发育背景下新的摄食结构的形态进化问题。 从薄切片进行比较性的高分辨率TEM重建对于解释形态演化是必不可少的。 高压冷冻和冷冻置换已被证明是最佳的，上级任何其他已知的方法保存线虫超微结构。 在此基础上，要求使用NCMR的Bal-Tec HPM 010高压冷冻机。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proposed research addresses questions of morphological evolution of novel feeding structures in plant parasitic nematodes within a phylogenetic context. Comparative, high resolution TEM reconstruction from thin sections is essential for interpreting morphological evolution. High pressure freezing and freeze substitution have been proven to be optimal and superior to any other known approach for preservation of nematode ultrastructure. On this basis, access to the Bal-Tec HPM 010 High Pressure Freezing Machine at NCMIR is requested.