IDENTIFICATION OF DEVELOPMENTAL MODULES IN CEPHALOBID NEMATODES

头孢线虫发育模块的鉴定

• 批准号: 7358072
• 负责人: J G BALDWIN
• 金额: $ 1.22万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358072
• 关键词: IDENTIFICATION; DEVELOPMENTAL; MODULES; CEPHALOBID; NEMATODES

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Members of the family Cephalobidae (Nematoda: Rhabditida) are non-parasitic microbial-feeding nematodes with cephalic cuticular appendages called probolae. They are nearly ubiquitously distributed in terrestrial ecosystems, yet very little is known of their biology. Probolae are the most important characters used for taxonomic purposes in the group, and their diversity and complexity makes them interesting models for evolutionary questions. They are complex extensions of the extracellular matrix, which adds further importance to understanding their morphology and morphogenesis. Preliminary work done in part at the NCMIR in 2001 suggests that electron tomography would prove invaluable for describing their complex 3D structure, including the relationship between up to seven different protein laminates of which probolae are composed. The work proposed here would contribute to a larger project which aims to formulate and test hypotheses of developmental modules that could be utilized to answer general questions about the origin of novel characters and the evolution of development. It will be combined with phylogenetic, developmental and SEM information in order to address theoretical evolutionary questions and provide a much needed description of anatomy in order to address issues concerning the homology of characters used in phylogenetic analyses and for diagnostic purposes. In addition to helping accomplish these research goals, the tomographic reconstruction will contain information on other sensory and feeding structures of significant interest to developmental geneticists and evolutionary biologists who use nematode models, and the work will serve to evaluate the technique for much broader application in understanding nematode anatomy. Access to a CM300 mid voltage microscope at the University of California, Riverside is already available, which would be used to preview material and to pre-expose sections to the electron beam. Preliminary work showed that contrast was the limiting factor for obtaining images of cuticular structures, so the new energy filtered microscope at the NCMIR would be used for acquiring the final tomographic series.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。头线虫科的成员（线虫纲：横纹肌目）是非寄生的微生物食性线虫，具有头状表皮附属物，称为尾虫。它们几乎无处不在地分布在陆地生态系统中，但对它们的生物学知之甚少。Probolae是类群中用于分类学目的的最重要的特征，它们的多样性和复杂性使它们成为研究进化问题的有趣模型。它们是细胞外基质的复杂延伸，这进一步增加了理解它们的形态和形态发生的重要性。2001年在NCMIR部分完成的初步工作表明，电子断层扫描将证明在描述它们复杂的3D结构方面是无价的，包括构成问题的多达7种不同蛋白质层板之间的关系。这里提出的工作将有助于一个更大的项目，该项目旨在制定和测试发展模块的假设，这些假设可用于回答有关小说人物起源和发展演变的一般问题。它将与系统发育、发育和扫描电镜信息相结合，以解决理论进化问题，并提供急需的解剖学描述，以解决系统发育分析和诊断目的中有关特征同源性的问题。除了帮助完成这些研究目标外，断层扫描重建还将包含对使用线虫模型的发育遗传学家和进化生物学家非常感兴趣的其他感觉和摄食结构的信息，并且这项工作将有助于评估该技术在理解线虫解剖学方面的更广泛应用。加州大学河滨分校（University of California, Riverside）的CM300中压显微镜已经可以使用，它将用于预览材料，并将切片预先暴露在电子束下。初步工作表明，对比度是获得角质层结构图像的限制因素，因此将使用NCMIR的新型能量过滤显微镜获得最终的层析序列。