COMPARATIVE TEM RECONSTRUCTION OF NEMATODE FEEDING STRUCTURES

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。拟议的研究解决了植物寄生线虫在系统发育背景下的新摄食结构的形态进化问题。比较，高分辨率透射电镜重建薄切片是必不可少的解释形态演变。高压冷冻和冷冻替代已被证明是保存线虫超微结构的最佳方法。在此基础上，要求使用核核区的Bal-Tec HPM 010高压冷冻机。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proposed research addresses questions of morphological evolution of novel feeding structures in plant parasitic nematodes within a phylogenetic context. Comparative, high resolution TEM reconstruction from thin sections is essential for interpreting morphological evolution. High pressure freezing and freeze substitution have been proven to be optimal and superior to any other known approach for preservation of nematode ultrastructure. On this basis, access to the Bal-Tec HPM 010 High Pressure Freezing Machine at NCMIR is requested.