COMPARATIVE TEM RECONSTRUCTION OF NEMATODE FEEDING STRUCTURES

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。拟议的研究在系统发育的背景下解决了植物寄生线虫中新的摄食结构的形态进化问题。比较、高分辨率的薄片透射电子显微镜重建对于解释形态演化是必不可少的。高压冷冻和冷冻替代已被证明是保存线虫超微结构的最佳和优于任何其他已知的方法。在此基础上，申请使用NCMIR的Bal-Tec HPM 010高压冷冻机。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proposed research addresses questions of morphological evolution of novel feeding structures in plant parasitic nematodes within a phylogenetic context. Comparative, high resolution TEM reconstruction from thin sections is essential for interpreting morphological evolution. High pressure freezing and freeze substitution have been proven to be optimal and superior to any other known approach for preservation of nematode ultrastructure. On this basis, access to the Bal-Tec HPM 010 High Pressure Freezing Machine at NCMIR is requested.