COMPARATIVE TEM RECONSTRUCTION OF NEMATODE FEEDING STRUCTURES

线虫摄食结构的比较TEM重建

• 批准号: 8169611
• 负责人: J G BALDWIN
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169611
• 关键词: Address; Biological Preservation; Computer Retrieval of Information on Scientific Projects Database; Evolution; Freeze Substitution; Freezing; Funding; Grant; Institution; Microtomy; Nematoda; Parasitic nematode; Phylogenetic Analysis; Plants; Research; Research Personnel; Resolution; Resources; Source; Structure; United States National Institutes of Health; base; comparative; feeding; novel; pressure; reconstruction

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proposed research addresses questions of morphological evolution of novel feeding structures in plant parasitic nematodes within a phylogenetic context. Comparative, high resolution TEM reconstruction from thin sections is essential for interpreting morphological evolution. High pressure freezing and freeze substitution have been proven to be optimal and superior to any other known approach for preservation of nematode ultrastructure. On this basis, access to the Bal-Tec HPM 010 High Pressure Freezing Machine at NCMIR is requested.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 拟议的研究解决了系统发育背景下植物寄生线虫新型摄食结构的形态进化问题。 从薄片进行比较、高分辨率 TEM 重建对于解释形态演化至关重要。 高压冷冻和冷冻替代已被证明是最佳的，并且优于任何其他已知的线虫超微结构保存方法。 在此基础上，请求访问 NCMIR 的 Bal-Tec HPM 010 高压冷冻机。