COMPARATIVE TEM RECONSTRUCTION OF NEMATODE FEEDING STRUCTURES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 拟议的研究在系统发育的背景下解决了植物寄生线虫中新的摄食结构的形态进化问题。比较、高分辨率的薄片透射电子显微镜重建对于解释形态演化是必不可少的。高压冷冻和冷冻替代已被证明是保存线虫超微结构的最佳和优于任何其他已知的方法。在此基础上，申请使用NCMIR的Bal-Tec HPM 010高压冷冻机。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proposed research addresses questions of morphological evolution of novel feeding structures in plant parasitic nematodes within a phylogenetic context. Comparative, high resolution TEM reconstruction from thin sections is essential for interpreting morphological evolution. High pressure freezing and freeze substitution have been proven to be optimal and superior to any other known approach for preservation of nematode ultrastructure. On this basis, access to the Bal-Tec HPM 010 High Pressure Freezing Machine at NCMIR is requested.