ENGINEERING OF BICELLES FOR PULSED DIPOLAR ESR STUDY ON MEMBRANE PROTEINS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 双胞提供了一种环境，其中蛋白质可以与由生物活性脂质组成的双层结构相互作用，包装成足够小的粒度，以允许在溶液中快速翻滚时间用于NMR，并在脉冲偶极ESR光谱中使用的冷冻样品中获得高蛋白质浓度和均匀分布。因此，有必要设计合适的双胞，并探索其在蛋白质-膜相互作用研究中的应用。我们追求以下具体目标：（1）制备作为生物膜模拟物的双胞，基于不同的长链和短链脂质的组成和比例;（2）表征它们的大小和相对于它们与蛋白质的负载的稳定性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bicelles provide an environment wherein proteins can interact with a bilayer structure composed of biologically competent lipids, packaged into a particle size that is sufficiently small to allow for rapid tumbling times in solution for NMR and for high protein concentrations and uniform distributions in the frozen samples used in pulsed dipolar ESR spectroscopy. According to this, it was necessary to engineer suitable bicelles and explore their utility for studies of protein-membrane interactions. We pursued the following specific goal: (1) to prepare bicelles as biological membrane mimetics, based on different compositions and ratios of long chain to short chain lipids; (2) to characterize their size and stability with respect to their loading with protein.