MODEL PROTEIN PRODUCTION FOR TIME-RESOLVED 2D-ELDOR, PDS, AND MQC ESR

时间分辨 2D-ELDOR、PDS 和 MQC ESR 的蛋白质生产模型

• 批准号: 8172240
• 负责人: ELKA R GEORGIEVA
• 金额: $ 0.96万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172240
• 关键词: Biological Models; Calmodulin; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Coupled; DNA; Deer; Development; Funding; Grant; Housing; Institution; Methods; Modeling; Muramidase; Production; Proteins; Research; Research Personnel; Resources; Sampling; Source; Spectrum Analysis; Spin Labels; Time; United States National Institutes of Health; interest

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Good model systems are needed to develop methods, which is certainly true of PDS. Long organic biradicals, polyradicals, and supramolecular assemblies were instrumental for development of DEER. However they are difficult to synthesize and require expertise of a good organic chemist, who should be interested in such collaborations. In our development of DQC method we could not rely on this but made a good use of few available biradicals with distances in the range of 10-30 ¿. But when we needed a longer distances, we had to reach to Gunnar Jeschke for the sample. However we did find that for example DNA duplexes or some proteins can also contribute good model systems and are relatively easy to produce in house in required quantities. For the project development on time-resolved 2D-ELDOR spectroscopy, coupled with stopped flow or continuous rapid flow, we require large amounts (up to grams) of spin-labeled model proteins. For example T4 Lysozyme and Calmodulin are relatively small and stable proteins that can be expressed in required quantities with a moderate effort. In general proteins and DNA could provide us with all required distances and a variety of spin configurations. Furthermore, they are much more relevant to our research.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 开发方法需要良好的模型系统，PDS当然也是如此。长的有机双自由基、多自由基和超分子组装对鹿的发育起着重要的作用。然而，它们很难合成，需要优秀的有机化学家的专业知识，他们应该对这种合作感兴趣。在我们的DQC方法的发展中，我们不能依赖于此，而是很好地利用了几个可用的双根，距离在10-30°之间。但当我们需要更长的距离时，我们不得不联系冈纳尔·杰施克来获取样本。然而，我们确实发现，例如，DNA双链或某些蛋白质也可以贡献良好的模型系统，并且相对容易在内部生产所需的数量。对于时间分辨2D-Eldor光谱学的项目开发，再加上停止流动或连续快速流动，我们需要大量(高达克)的自旋标记模型蛋白质。例如，T4溶菌酶和钙调蛋白是相对较小和稳定的蛋白质，可以适度地表达所需的数量。总的来说，蛋白质和DNA可以为我们提供所有所需的距离和各种自旋构型。此外，它们与我们的研究更相关。