flow cytometry

流式细胞仪

• 批准号: 7339465
• 负责人: Charles L. Goolsby
• 金额: $ 14.76万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7339465
• 关键词: Accounting; Biological Assay; Cancer Center; Cell Line; Cell Separation; Cells; Clinical Sciences; Clinical Trials; Color; Complex; Comprehensive Cancer Center; Consultations; Core Facility; Cytometry; DNA analysis; Data Analyses; Data Quality; Disease; Equipment; Experimental Designs; Extrasensory Perception; Flow Cytometry; Focus Groups; Generations; Goals; Heterogeneity; Housing; Human Resources; Immunophenotyping; Individual; Institution; Laboratories; Lasers; Malignant Neoplasms; Measurement; Numbers; Operative Surgical Procedures; Preparation; Publishing; Quality Control; Research; Research Personnel; Sampling; Services; Sorting - Cell Movement; Speed; Training; Translational Research; Universities; assay development; base; cancer research center director; data acquisition; instrument; interest; medical schools; member; programs

The Robert H. Lurie Comprehensive Cancer Center Flow Cytometry Core Facility provides comprehensive flow cytometry and cell sorting services. The Flow Cytometry Core Facility serves investigators of the Cancer Center, Northwestern University Medical School, Northwestern University and other affiliated institutions by providing access to routine flow cytometry assays such as immunophenotyping and DNA analysis. However, research users encompass individuals with a broad spectrum of training in the basic and clinical sciences, and thus, a major goal of the facility is to provide the guidance, technical assistance, and equipment for investigators to utilize more complex multi-parametric, multi-laser measurements as well as cell sorting in their research regardless of their level of cytometry expertise. The equipment and expertise housed within the Core Facility permits implementation of most flow cytometry assays that have been published. Services provided by the facility personnel extend from consultation on experimental design, sample preparation and data analysis to instrument operation and set-up for cell-sorting and multi-laser operation. Because of the emphasis the facility places on the quality of data, the flow laboratory also offers services for sample preparation, data acquisition, and data analysis. In addition, the Core Facility has an active program of assay development guided by the interests of the investigators. As such, the Flow Cytometry Core Facility serves as a focus for individuals interested in cellular based measurements and cellular heterogeneity in disease providing critical support for cancer related research within the institution. This is also reflected by the Core Director's, and Cancer Center's, key founding involvement in the Cytometry Consortium, a multiinstitutional collaborative group focusing on complex multi-parametric cell based cytometry assay development in support of clinical trials and translational research. The facility includes two cell sorters; a five laser, 13 color MoFlo high speed cell sorter (Dako) and a two laser, six color Elite ESP (Beckman Coulter) cell sorter. In addition the facility has two benchtop flow cytometry analyzers; a 3 laser, 9 color Cyan (Dako) and a single laser, four color XL (Beckman Coulter). In addition, the flow lab has a ViCell cell .: counter/viability measurement instrument (Beckman Coulter) for cell line characterization and quality control for high speed sorting. In support of these instruments, the facility also has an offline analysis workstation providing the components for post acquisition data analysis and graphics generation. The Core Facility is now heavily utilized by a wide number of investigators (88), approximately 64.7% of which are Cancer Center members who account for approximately 73.0% of core utilization.

罗伯特·H。Lurie综合癌症中心流式细胞术核心设施提供全面的 流式细胞仪和细胞分选服务。流式细胞术核心设施为癌症研究者提供服务， 中心、西北大学医学院、西北大学等附属机构 提供常规流式细胞术分析，如免疫表型分析和DNA分析。然而，在这方面， 研究用户包括在基础和临床科学方面受过广泛培训的个人， 因此，该设施的一个主要目标是提供指导、技术援助和设备， 研究人员利用更复杂的多参数，多激光测量以及细胞分选， 他们的研究，无论他们的水平细胞计数的专业知识。里面的设备和专业知识 核心设施允许实施已经公布的大多数流式细胞术测定。服务 由机构工作人员提供的实验设计、样品制备和 数据分析到仪器操作和细胞分选和多激光器操作的设置。因为 流量实验室强调数据的质量，还提供样品分析服务。 准备、数据采集和数据分析。此外，核心基金还有一个积极的方案， 以研究者的兴趣为导向的分析开发。因此，流式细胞术核心设施 作为对基于细胞的测量和细胞异质性感兴趣的个人的焦点， 为机构内的癌症相关研究提供关键支持。这也反映在 核心主任和癌症中心在细胞测量联盟中的关键创始参与，这是一个多机构的组织。 专注于基于复杂多参数细胞的细胞计数测定的协作组 支持临床试验和转化研究的发展。该设施包括两个细胞分选机;一个五 激光，13色MoFlo高速细胞分选仪（Dako）和双激光，六色Elite ESP（Beckman Coulter） 细胞分类器此外，该机构还有两台台式流式细胞仪;一台3激光，9色青色（Dako） 和一台四色XL激光器（Beckman Coulter）。此外，流动实验室还有ViCell细胞。 用于细胞系表征和质量控制的计数器/活力测量仪器（Beckman Coulter） 用于高速分拣。为了支持这些仪器，该设施还设有离线分析工作站 提供用于采集后数据分析和图形生成的组件。核心设施是 现在被大量的研究者（88）大量使用，其中约64.7%是癌症患者 约占核心利用率73.0%的中心成员。