FACSAria SORP Cell Sorter

描述（由申请人提供）：罗伯特H。Lurie综合癌症中心（RHLCCC）流式细胞术核心设施是一个成熟的、多产的、高度互动的和维护良好的最先进的设施，为研究者提供成功完成几乎任何流式细胞术实验所需的所有服务水平。重点放在用户教育/互动和实验优化。由研究者需求驱动的灵活服务水平范围从咨询、样品制备、数据采集/分析和出版物图形生成到简单地提供优化的仪器。主要的RHLCCC流式细胞仪核心设施位于医学院校园内，拥有六台分析细胞仪，包括最先进的6台BD LSRFortessa激光细胞仪（详情如下）。整个园区的所有分拣需求都是在2003年购买的5激光Beckman-Coulter MoFlo仪器上完成的。尽管MoFlo是一种功能强大、使用频繁的仪器，但其设计过时，性能和功能无法很好地满足用户当前不断变化的复杂分析需求。该设施最先进的分析能力提供了MoFlo无法实现的激发线和灵敏度。此外，过时的MoFlo设计在许多情况下会损害细胞回收。其次，部分由于正在进行的程序化调查扩展，分拣用户群扩大了10倍以上。相对于分析结果和能力的MoFlo能力阻碍了用户满意度，在许多情况下也阻碍了科学进步。最后，快速增加的转化研究重点突出了对人类细胞安全，生物包含分选能力的需求，超出了我们目前MoFlo的范围。本提案旨在购买FACSAria SORP，台式高速细胞分选仪，配备5个激光器（405、488、552、640和690 nm），能够分析多达11个不同的参数，以及前向和侧向散射、气溶胶密闭室和温度调节功能。分选机将永久放置在Baker BioProtect IV-LE生物安全罩中。如果获得资助，这将提供最先进的细胞分选仪仪器，具有安全的人类细胞分选能力，以满足我们用户群日益复杂的需求。

DESCRIPTION (provided by applicant): The Robert H. Lurie Comprehensive Cancer Center (RHLCCC) Flow Cytometry Core Facility is an established, productive, highly interactive and well maintained state of the art facility offering investigators all service levels needed for successful completion of virtually any flow cytometry experiment. Strong emphasis is placed on user education/interaction and experimental optimization. Flexible service levels driven by investigator needs range from consultation, sample preparation, data acquisition/analysis and publication graphic generation to simply providing optimized instrumentation. The main RHLCCC Flow Cytometry Core Facility is on the Medical School campus and has six analytical cytometers including a state of the art 6 laser BD LSRFortessa (details below). All sorting demands for the entire campus are accomplished on a 5 laser Beckman-Coulter MoFlo instrument purchased in 2003. Although a robust, heavily utilized instrument, the MoFlo design is dated with performance and capabilities not well matched to current evolving complex analytical demands of users. The facility's state of the art analytical capabilities provide excitation lines and sensitivity not achievable on the MoFlo. Further, the dated MoFlo design compromises cell recovery in many instances. Secondly, in part due to ongoing programmatic investigator expansion, sorter user base has expanded more than 10 fold. Both MoFlo capabilities relative to analytical results and capacity have hindered user satisfaction and in many cases scientific progress. Lastly, rapidly increasing translational research emphasis has accentuated the need for safe, bio-contained sorting capabilities of human cells beyond the scope of our current MoFlo. This proposal is for purchase of a FACSAria SORP, bench-top high speed cell sorter equipped with five lasers (405, 488, 552, 640, and 690nm) capable of analyzing up to 11 different parameters plus forward and side scatter, aerosol containment chamber, and temperature regulation features. The sorter will be permanently housed in a Baker BioProtect IV-LE biosafety hood. If funded, this will provide state-of-the-art cell sorter instrumentation with safe human cell sorting capabilities to meet the increasingly complex needs of our user base.