FACSAria SORP Cell Sorter

描述(由申请人提供)：Robert H.Lurie综合癌症中心(RHLCCC)流式细胞术核心设施是一家成熟、高效、高度互动和维护良好的设施，为研究人员提供成功完成几乎任何流式细胞术实验所需的所有服务水平。非常重视用户教育/互动和实验优化。由调查人员需求驱动的灵活服务水平从咨询、样品准备、数据采集/分析和出版物图形生成到简单地提供优化的仪器。主要的RHLCCC流式细胞仪核心设施位于医学院校园内，拥有六台分析细胞仪，其中包括一台最先进的6激光BD LSRFortessa(详细信息如下)。整个校园的所有分拣要求都是在2003年购买的5激光Beckman-Coulter MoFlo仪器上完成的。虽然MoFlo是一种坚固耐用、使用率很高的仪器，但它的性能和功能与当前用户不断发展的复杂分析需求不能很好地匹配。该设施的最先进的分析能力提供了MoFlo无法实现的激发线和灵敏度。此外，过时的MoFlo设计在许多情况下损害了细胞回收。其次，部分由于正在进行的方案调查员扩展，分类器的用户基础扩大了10倍以上。MoFlo相对于分析结果的能力和能力都阻碍了用户的满意，在许多情况下还阻碍了科学进步。最后，迅速增加的对翻译研究的重视加剧了对人类细胞安全、生物包含的分选能力的需求，超出了我们目前的MoFlo的范围。该建议书用于购买FACSAria SORP台式高速细胞分选机，配备五种激光(405、488、552、640和690 nm)，能够分析多达11个不同的参数以及正向和侧向散射、气溶胶密封室和温度调节功能。分拣机将被永久安置在贝克生物保护IV-LE生物安全罩中。如果获得资金，这将提供最先进的细胞分选仪器，具有安全的人体细胞分选能力，以满足我们用户群日益复杂的需求。

DESCRIPTION (provided by applicant): The Robert H. Lurie Comprehensive Cancer Center (RHLCCC) Flow Cytometry Core Facility is an established, productive, highly interactive and well maintained state of the art facility offering investigators all service levels needed for successful completion of virtually any flow cytometry experiment. Strong emphasis is placed on user education/interaction and experimental optimization. Flexible service levels driven by investigator needs range from consultation, sample preparation, data acquisition/analysis and publication graphic generation to simply providing optimized instrumentation. The main RHLCCC Flow Cytometry Core Facility is on the Medical School campus and has six analytical cytometers including a state of the art 6 laser BD LSRFortessa (details below). All sorting demands for the entire campus are accomplished on a 5 laser Beckman-Coulter MoFlo instrument purchased in 2003. Although a robust, heavily utilized instrument, the MoFlo design is dated with performance and capabilities not well matched to current evolving complex analytical demands of users. The facility's state of the art analytical capabilities provide excitation lines and sensitivity not achievable on the MoFlo. Further, the dated MoFlo design compromises cell recovery in many instances. Secondly, in part due to ongoing programmatic investigator expansion, sorter user base has expanded more than 10 fold. Both MoFlo capabilities relative to analytical results and capacity have hindered user satisfaction and in many cases scientific progress. Lastly, rapidly increasing translational research emphasis has accentuated the need for safe, bio-contained sorting capabilities of human cells beyond the scope of our current MoFlo. This proposal is for purchase of a FACSAria SORP, bench-top high speed cell sorter equipped with five lasers (405, 488, 552, 640, and 690nm) capable of analyzing up to 11 different parameters plus forward and side scatter, aerosol containment chamber, and temperature regulation features. The sorter will be permanently housed in a Baker BioProtect IV-LE biosafety hood. If funded, this will provide state-of-the-art cell sorter instrumentation with safe human cell sorting capabilities to meet the increasingly complex needs of our user base.