FACSAria SORP Cell Sorter

描述（由申请人提供）：Robert H. Lurie综合癌症中心（RHLCCC）流式细胞术核心设施是一个成熟的，高效的，高度互动的，维护良好的最先进的设施，为研究人员提供成功完成几乎任何流式细胞术实验所需的所有服务水平。重点放在用户教育/交互和实验优化。灵活的服务水平由研究者的需求驱动，从咨询，样品制备，数据采集/分析和出版图形生成到简单地提供优化的仪器。主要的RHLCCC流式细胞仪核心设施位于医学院校园内，拥有六台分析细胞仪，包括一台最先进的6激光BD LSRFortessa（详情如下）。整个校区的所有分选需求都是在2003年购买的5激光Beckman-Coulter MoFlo仪器上完成的。虽然MoFlo是一种强大的、被广泛使用的仪器，但它的设计已经过时，性能和功能不能很好地满足当前用户不断发展的复杂分析需求。该设备最先进的分析能力提供了MoFlo无法实现的激励线和灵敏度。此外，过时的MoFlo设计在许多情况下会影响细胞恢复。其次，部分由于正在进行的程序化调查员扩展，分类器的用户基础扩大了10倍以上。MoFlo在分析结果和容量方面的能力都阻碍了用户满意度，在许多情况下也阻碍了科学进步。最后，快速增长的转化研究重点强调了对安全的、生物含量的人类细胞分选能力的需求，超出了我们目前MoFlo的范围。该提案是购买FACSAria SORP，台式高速细胞分选机，配备五种激光器（405、488、5552、640和690nm），能够分析多达11种不同的参数，以及前向和侧向散射、气溶胶密封室和温度调节功能。分选机将永久安置在Baker BioProtect IV-LE生物安全罩中。如果获得资助，这将提供最先进的细胞分选仪器，具有安全的人类细胞分选能力，以满足我们用户群日益复杂的需求。

DESCRIPTION (provided by applicant): The Robert H. Lurie Comprehensive Cancer Center (RHLCCC) Flow Cytometry Core Facility is an established, productive, highly interactive and well maintained state of the art facility offering investigators all service levels needed for successful completion of virtually any flow cytometry experiment. Strong emphasis is placed on user education/interaction and experimental optimization. Flexible service levels driven by investigator needs range from consultation, sample preparation, data acquisition/analysis and publication graphic generation to simply providing optimized instrumentation. The main RHLCCC Flow Cytometry Core Facility is on the Medical School campus and has six analytical cytometers including a state of the art 6 laser BD LSRFortessa (details below). All sorting demands for the entire campus are accomplished on a 5 laser Beckman-Coulter MoFlo instrument purchased in 2003. Although a robust, heavily utilized instrument, the MoFlo design is dated with performance and capabilities not well matched to current evolving complex analytical demands of users. The facility's state of the art analytical capabilities provide excitation lines and sensitivity not achievable on the MoFlo. Further, the dated MoFlo design compromises cell recovery in many instances. Secondly, in part due to ongoing programmatic investigator expansion, sorter user base has expanded more than 10 fold. Both MoFlo capabilities relative to analytical results and capacity have hindered user satisfaction and in many cases scientific progress. Lastly, rapidly increasing translational research emphasis has accentuated the need for safe, bio-contained sorting capabilities of human cells beyond the scope of our current MoFlo. This proposal is for purchase of a FACSAria SORP, bench-top high speed cell sorter equipped with five lasers (405, 488, 552, 640, and 690nm) capable of analyzing up to 11 different parameters plus forward and side scatter, aerosol containment chamber, and temperature regulation features. The sorter will be permanently housed in a Baker BioProtect IV-LE biosafety hood. If funded, this will provide state-of-the-art cell sorter instrumentation with safe human cell sorting capabilities to meet the increasingly complex needs of our user base.