MoFlo High Speed Multilaser Flow Cytometer/Cell Sorter

描述（由申请人提供）：流式细胞术已成为一种关键和关键的研究工具，在基础研究、临床转化研究和临床研究的广泛学科中应用。目前，已经有大量的试剂用于鉴定特定的细胞亚群，研究调节细胞增殖、凋亡、信号转导途径和细胞功能状态的分子。此外，荧光染料和标记技术现在可用于允许同时检测单个细胞中的许多这些分子和标记物。这导致需要越来越复杂的细胞计数分析，以了解这些分子在细胞水平上的作用和相互作用。流式细胞仪的核心设施。西北大学Lurie综合癌症中心为59名研究人员提供服务，国家同行评审资金为22，631，071美元。该机构与其用户群有着密切、积极的互动，促进了所有研究者（无论其专业水平如何）实施更困难的流式细胞术测定。再加上可用试剂的增加，对该设施的要求越来越复杂。目前，该设施有一台台式分析仪，一台Beckman-Coulter XLMLC四色仪器和一台使用六年多的Beckman-Coulter ELITE ESP细胞分选仪。目前的细胞分选机在复杂、多激光和高速分选应用领域具有明显的局限性。流式细胞仪核心设备满足我们用户群当前和未来需求的关键是开发更复杂和先进的多参数和高速分选能力。所要求的MoFlo（TM）仪器将促进这一关键的发展。MoFlo增强的多激光器功能（三个独立的激光询问点，所有荧光检测器的聚焦/针孔收集）和高速分选能力（25，000 - 50，000个细胞/秒，10- 15倍于我们目前的仪器能力）将有助于这一发展。此外，主管Charles Goolsby博士（在研究和临床流式细胞术方面拥有23年以上的经验）和玛丽帕尼亚瓜MT（ASCP）SI（在流式细胞术方面拥有12年以上的经验）对设施的监督确保了这些增强功能的有效实施。

DESCRIPTION (provided by applicant): Flow cytometry has become a key and critical research tool with applications in a wide range of disciplines in basic research, clinical translational research, and clinical studies. A wealth of reagents to identify specific cell sub-population, to study molecules regulating cell proliferation, apoptosis, signal transduction pathways, and functional states in cells are now available. Further, the fluorescent dyes and labeling techniques are now available to permit the simultaneous detection of many of these molecules and markers in individual cells. This is leading to the need for increasingly complex cytometric assays to understand the role and interaction of these molecules at the cell level. The Flow Cytometry Core Facility of the Robert H. Lurie Comprehensive Cancer Center at Northwestern University serves over 59 investigators with national peer reviewed funding of $22,631,071. This facility has a strong history of close, active interaction with its user base facilitating implementation of more difficult flow cytometry assays by all investigators regardless of their level of expertise. This coupled with the increase in available reagents has fostered increasingly complex requests of the facility. At present, the facility has a bench top analyzer, a Beckman-Coulter XLMLC four color instrument and a Beckman-Coulter ELITE ESP cell sorter which is over six years old. The current cell sorter has distinct limitations in the areas of complex, multi-laser and high speed sorting applications. Key for the Flow Cytometry Core Facility to meet the current and future needs of our user base is development of more complex and sophisticated multi-parameter and high-speed sorting capabilities. The requested MoFlo(TM) instrumentation will facilitate this critical development. This development will be aided by the MoFlo's enhanced multi-laser capabilities (three separate laser interrogation spots, focused/pinhole collection for all fluorescence detectors) and high speed sorting capabilities (25,000-50,000 cells/sec, 10-15X our current instrument capabilities). Further, the facilities oversight by Director, Dr. Charles Goolsby, with over 23 years experience in research and clinical flow cytometry and Mary Paniagua MT(ASCP)SI, with over 12 years experience in flow cytometry ensure that efficient implementation of these enhanced capabilities will occur.