MoFlo High Speed Multilaser Flow Cytometer/Cell Sorter

描述(申请人提供)：流式细胞术已成为一个关键和关键的研究工具，在基础研究、临床转化研究和临床研究的广泛学科中应用。现在已经有了大量的试剂来识别特定的细胞亚群，研究调控细胞增殖、凋亡、信号转导途径和细胞功能状态的分子。此外，荧光染料和标记技术现在可以用来在单个细胞中同时检测许多这样的分子和标记。这导致了对日益复杂的细胞分析的需要，以了解这些分子在细胞水平上的作用和相互作用。西北大学罗伯特·H·鲁里综合癌症中心的流式细胞术核心设施为59名研究人员提供全国同行评审的22,631,071美元的资金。该设施与其用户群有着密切、积极的互动的强大历史，促进了所有研究人员实施更困难的流式细胞仪分析，无论他们的专业水平如何。这一点，再加上可用试剂的增加，导致对该设施的要求越来越复杂。目前，该设施拥有一台台式分析仪，一台Beckman-Coulter XLMLC四色仪和一台Beckman-Coulter Elite ESP细胞分选机，已有六年多的历史。目前的细胞分选机在复杂、多激光和高速分选应用领域有明显的局限性。Flow细胞仪核心设备满足我们用户群当前和未来需求的关键是开发更复杂和尖端的多参数和高速分选能力。所要求的MoFlo(TM)仪器将促进这一关键开发。这一发展将得益于MoFlo增强的多激光能力(三个独立的激光询问点，所有荧光探测器的聚焦/针孔收集)和高速分选能力(25,000-50,000个细胞/秒，是我们当前仪器能力的10-15倍)。此外，拥有超过23年研究和临床流式细胞术经验的主管Charles Goolsby博士和拥有超过12年的流式细胞仪经验的Mary Paniawa MT(ASCP)SI的设施监督确保了这些增强能力的有效实施。

DESCRIPTION (provided by applicant): Flow cytometry has become a key and critical research tool with applications in a wide range of disciplines in basic research, clinical translational research, and clinical studies. A wealth of reagents to identify specific cell sub-population, to study molecules regulating cell proliferation, apoptosis, signal transduction pathways, and functional states in cells are now available. Further, the fluorescent dyes and labeling techniques are now available to permit the simultaneous detection of many of these molecules and markers in individual cells. This is leading to the need for increasingly complex cytometric assays to understand the role and interaction of these molecules at the cell level. The Flow Cytometry Core Facility of the Robert H. Lurie Comprehensive Cancer Center at Northwestern University serves over 59 investigators with national peer reviewed funding of $22,631,071. This facility has a strong history of close, active interaction with its user base facilitating implementation of more difficult flow cytometry assays by all investigators regardless of their level of expertise. This coupled with the increase in available reagents has fostered increasingly complex requests of the facility. At present, the facility has a bench top analyzer, a Beckman-Coulter XLMLC four color instrument and a Beckman-Coulter ELITE ESP cell sorter which is over six years old. The current cell sorter has distinct limitations in the areas of complex, multi-laser and high speed sorting applications. Key for the Flow Cytometry Core Facility to meet the current and future needs of our user base is development of more complex and sophisticated multi-parameter and high-speed sorting capabilities. The requested MoFlo(TM) instrumentation will facilitate this critical development. This development will be aided by the MoFlo's enhanced multi-laser capabilities (three separate laser interrogation spots, focused/pinhole collection for all fluorescence detectors) and high speed sorting capabilities (25,000-50,000 cells/sec, 10-15X our current instrument capabilities). Further, the facilities oversight by Director, Dr. Charles Goolsby, with over 23 years experience in research and clinical flow cytometry and Mary Paniagua MT(ASCP)SI, with over 12 years experience in flow cytometry ensure that efficient implementation of these enhanced capabilities will occur.