Stimulation of tooth repair and regeneration by interaction with dental meterials

通过与牙科材料的相互作用刺激牙齿修复和再生

• 批准号: 7847114
• 负责人: Jack L. Ferracane
• 金额: $ 0.87万
• 依托单位: UNIVERSITY OF BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-23 至 2010-06-22
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7847114
• 关键词: Stimulation; tooth; repair; regeneration; interaction

DESCRIPTION (provided by applicant): The overall goal of this application is to provide the candidate, a senior investigator with an extensive background in dental materials research, with experience in the techniques used to study the regeneration of living dental tissues, pulp and dentin, as a result of injury or insult from dental disease and their subsequent interactions with dental materials. Advances in regenerative medicine are paving the way for a new era in restorative dentistry and this training will position the candidate to be a key player in contributing to this field. This experience, including the specific research project, are in support of the goals of the NIDCR which is to encourage research on the repair and regeneration of orofacial tissues, to determine the safety of commonly used dental materials, and to provide an adequate and well-trained research workforce. To accomplish the overall goal, a series of experiments have been designed by the candidate, with the advice of the sponsor, Dr. Tony Smith (U. Birmingham), an expert in this field. Dentin adhesives and glass ionomer restoratives, with and without a bioactive glass additive, will serve as the test materials. The research plan for this senior fellowship application has three specific aims. The first aim is to treat human dentin with acid of systematically greater strength (lower pH), representing differences in current "selfetching" dental adhesives, as well as commercial versions of self-etching adhesives and conventional glass ionomer, and to identify and quantitate the bioactive molecules, such as growth factors, that are released. The second aim is to use cell culture and gene expression to examine the viability of the bioactive molecules released from dentin by the various materials in terms of their ability to stimulate dentin regeneration. The third aim is to use the tooth slice organ culture model developed at the University of Birmingham to study the extent of cell death and the healing process of dentin exposed to specific dental materials; and to determine if the healing process can be enhanced by incorporating into the self-etching adhesive and glass ionomer a calcium and phosphate releasing material, a silica-based bioactive glass, that is currently being studied by the applicant for use in dental adhesives and restoratives. Public Health Relevance: This work is relevant to public health in that it investigates the manner in which tooth tissues respond to materials used to restore teeth and repair and regenerate themselves. This work also seeks to identify dental materials that may aid in the healing process by releasing the tooth's own molecules needed for the repair or regeneration process.

描述（由申请人提供）：本申请的总体目标是为申请人提供一名在牙科材料研究方面具有广泛背景的高级研究员，具有用于研究因牙科疾病造成的损伤或侮辱以及随后与牙科材料相互作用而导致的活牙组织、牙髓和牙本质再生的技术经验。再生医学的进步正在为修复牙科的新时代铺平道路，该培训将使候选人成为该领域的关键人物。这些经验，包括具体的研究项目，都支持NIDCR的目标，即鼓励对口腔面部组织的修复和再生的研究，确定常用牙科材料的安全性，并提供足够的和训练有素的研究人员。为了实现总体目标，候选人在赞助人、该领域专家托尼·史密斯博士（U. Birmingham）的建议下设计了一系列实验。牙本质粘接剂和玻璃离子修复剂，有或没有生物活性玻璃添加剂，将作为测试材料。这项高级奖学金申请的研究计划有三个具体目标。第一个目标是用系统强度更大（pH值更低）的酸处理人类牙本质，这代表了当前“自取”牙科粘合剂，以及自蚀刻粘合剂和传统玻璃离聚物的商业版本的差异，并确定和量化释放的生物活性分子，如生长因子。第二个目标是利用细胞培养和基因表达来检验各种材料从牙本质中释放的生物活性分子在刺激牙本质再生能力方面的可行性。第三个目标是利用伯明翰大学开发的牙片器官培养模型，研究暴露于特定牙科材料的牙本质的细胞死亡程度和愈合过程；并确定是否可以通过将钙和磷酸盐释放材料（硅基生物活性玻璃）加入自蚀刻粘合剂和玻璃离聚体来增强愈合过程，该材料目前正在由申请人研究用于牙科粘合剂和修复剂。公共卫生相关性：这项工作与公共卫生相关，因为它调查了牙齿组织对用于修复牙齿、修复和再生的材料的反应方式。这项工作还试图确定牙科材料，可以通过释放修复或再生过程所需的牙齿自身分子来帮助愈合过程。