Animal Care Core

动物护理核心

• 批准号: 7904081
• 负责人: DENISA D WAGNER
• 金额: $ 51.16万
• 依托单位: IMMUNE DISEASE INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7904081
• 关键词: Animal Care and Use Committees; Animal Experimentation; Animal Housing; Animal Model; Animals; Attention; Autopsy; Backcrossings; Biology; Blood; Blood Platelets; Blood specimen; Boston; Breeding; Caregivers; Cell Adhesion Molecules; Core Facility; DNA; Educational process of instructing; Engineering; Experimental Designs; Food Supply; Genetic; Genetic Engineering; Genotype; Grant; Health; Hemorrhage; Histology; Homing; House mice; Housing; Human Resources; Image; Individual; Injection of therapeutic agent; Institution; Investigation; Laboratories; Mus; Mutant Strains Mice; Partner in relationship; Procedures; Production; Protocols documentation; Records; Research; Research Personnel; Rest; Sentinel; Tail; Technology; Thrombus; Time; Training; Transfusion; Uncertainty; Veins; Water; Work; Writing; animal care; animal colony; college; computerized; cost; experience; member; mutant; programs; pup; research study; response; sex; tissue fixing

Animal models allowing investigation of the function of adhesion molecules in transfusion biology are one of the central themes of this program. Without any doubt the technology of genetic engineering of animals is one of the greatest advances in biology in recent years. Unfortunately, animal experimentation is not simple. In particular, there is variability: genetically engineered animals are not truly clones of each other and therefore their responses to treatment may differ. This is especially the case if the mice are of mixed background and not fully backcrossed to a particular genetic background. To minimize variability, wild-type animals are used from the same heterozygous matings to have litter mates comparisons or at least from the same animal colony. Therefore, often both mutant and wild-type animals have to be housed. So in addition to the mutant strains listed in Table 1, there are several wild-type strains that we need to keep. To obtain statistically meaningful results, each experiment has to involve a minimum of 6-10 animals of each genotype. The cost of animal housing is becoming prohibitive. This core unit is one of the reasons why this program was originally put together. To be able to maintain and breed a large number of mice of a certain genotype for experimental purposes, a mouse colony consisting of three breeding cages (to maintain the line) plus additional breeding and experimental cages (for expansion) is needed. In our experience a good ratio of breeding cages to experimental cages in the expansion colony is 1:6, commonly resulting in a colony of more than 50 cages. As Table 1 clearly shows this many colonies could not be established by each individual investigator and many mouse lines listed will be used in two or more different laboratories. Therefore, Stephen Cifuni, the animal technologist who will direct the mouse husbandry will be able to coordinate production of pups of one genotype for several laboratories in parallel. Working as a group will also minimize losses. Some experimental designs require a particular sex of the animals. The litter mates of the opposite sex will then most likely find use in another study at a different laboratory and will not have to be sacrificed. As the program developed and the projects expanded in their use of mice it became clear that not all mice now needed for Project 2 (Hartwig) and 4 (Silberstein) could be housed at CBRI. Therefore only their mice that will be used collaboratively at CBRI for imaging purposes, thrombus formation studies, platelet clearance, homing experiments etc will be housed within CBRI while the rest of the mice required for non -collaborative studies for these two projects will be housed in their own in-house facilities under subcontracts. The Core will continue to maintain strains that are used more rarely for all 4 projects. Mice produced at CBRI are easily transferred to the other institutions using the CBRI van and for this we keep a valid health certificate for our animals always on file. Dr. Wagner's laboratory members have trained a technologist, Stephen Cifuni, B.S. in Biology, Boston College, 2004. Mr. Cifuni has been employed at the Center for Blood Research for several months and has already been part of this program. He will now be directing the animal work in Core B. He is fully capable of managing the animal colonies that will continue to be part of this Core B. His duties are and continue to be the following 1) Check all cages first thing in the morning for water and food supplies, cleanliness and health of the animals. Correct all observed problems immediately and speak to care givers as necessary. 2) Mark cages that require veterinary attention with red cards and contact the veterinary consultant, Dr. Garibaldi, if necessary. 3) Make autopsies on dead animals and collect blood samples from sick animals for analysis. Have a kit ready to make cultures and be a veterinary helper as needed. 4) Send out sentinel animals and keep records. 5) Set up breedings on request and transport animals to laboratories. CBRI has a van that can be used if laboratory is located in another building. 6) Keep computerized records of all breedings and of all housed animals for every genotype. 7) Instruct all new program personnel on procedures and rules of the mouse facility - including the immuno-deficient room. 8) Keep records on budgetary issues, so that no group overspends the amount allocated to their project. 9) Actively participate in the Animal Care and Use Committee at CBRI, writing and reviewing animal protocols in particular those needed for this Program. Mr. Cifuni is also trained in special animal procedures such as retro-orbital bleeding; tailing mice for genotyping; PCR and Southern analysis of tail DNA; fixing tissues for histology; tail vein injections; etc. He is able to teach other members of the program these procedures

允许研究输血生物学中粘附分子功能的动物模型是其中之一 该计划的中心主题。毫无疑问，动物基因工程技术就是其中之一。 近年来生物学领域最伟大的进展。不幸的是，动物实验并不简单。在 特别是，存在变异性：基因工程动物并不是真正的彼此克隆，因此 他们对治疗的反应可能不同。如果小鼠具有混合背景并且 没有完全回交到特定的遗传背景。为了最大限度地减少变异性，使用野生型动物 来自相同的杂合交配以进行同窝交配比较或至少来自同一动物 殖民地。因此，通常必须饲养突变型和野生型动物。所以除了突变体 表1列出的菌株中，有几种我们需要保留的野生型菌株。统计得到 为了获得有意义的结果，每个实验必须至少涉及每种基因型的 6-10 只动物。 动物圈舍的成本正变得令人望而却步。这个核心单元是该计划得以成功的原因之一 原来放在一起的。能够维持和繁殖大量特定基因型的小鼠 实验目的，由三个饲养笼（以维持品系）组成的小鼠群体加上 需要额外的饲养和实验笼（用于扩展）。根据我们的经验，良好的比例 扩张群体中繁殖笼与实验笼的比例为1:6，通常会产生更多的群体 超过50个笼子。表 1 清楚地表明，每个个体不可能建立如此多的菌落 研究者和列出的许多小鼠品系将在两个或多个不同的实验室中使用。因此，斯蒂芬 Cifuni 是一位负责指导小鼠饲养的动物技术专家，他将能够协调生产 多个实验室同时获得一种基因型的幼崽。团队合作也能将损失降到最低。一些 实验设计需要特定的动物性别。然后，同窝的异性伴侣将会 最有可能在不同实验室的另一项研究中找到用途，并且不必牺牲。 随着该计划的发展和项目在小鼠使用方面的扩大，很明显，现在并不是所有的小鼠 项目 2 (Hartwig) 和 4 (Silberstein) 所需的设备可以安置在 CBRI。因此只有他们的老鼠才会 与 CBRI 合作用于成像目的、血栓形成研究、血小板清除、归巢 实验等将安置在 CBRI 内，而其余小鼠则用于非合作研究 这两个项目将根据分包合同安置在他们自己的内部设施中。核心将继续 维持所有 4 个项目中很少使用的菌株。 CBRI 生产的小鼠很容易转移到 使用 CBRI 货车的其他机构，为此，我们始终为我们的动物保留有效的健康证明 在文件上。 Wagner 博士的实验室成员培训了一位技术专家 Stephen Cifuni（理学学士）。波士顿学院生物学博士， 2004年，Cifuni先生在血液研究中心工作了几个月，并已 已成为该计划的一部分。他现在将指导 Core B 的动物工作。他完全有能力管理 动物群体将继续成为核心 B 的一部分。他的职责是并将继续是以下 1) 早上第一件事就是检查所有笼子的水和食物供应、动物的清洁和健康状况。 立即纠正所有观察到的问题，并根据需要与护理人员交谈。 2) 用红卡标记需要兽医注意的笼子，并联系兽医顾问 Dr. 加里波第，如果有必要的话。 3) 对死亡动物进行尸检并采集患病动物的血样进行分析。准备好套件 创造文化并根据需要成为兽医助手。 4）派出哨点动物并做好记录。 5) 根据要求进行饲养并将动物运送到实验室。 CBRI 有一辆货车，可以在以下情况下使用： 实验室位于另一栋大楼内。 6) 保存每种基因型的所有繁殖和所有圈养动物的计算机化记录。 7) 指导所有新项目人员了解小鼠设施的程序和规则 - 包括 免疫缺陷室。 8) 保留预算问题的记录，以便任何团体都不会超支分配给其项目的金额。 9) 积极参与CBRI动物护理和使用委员会，撰写和审查动物协议 特别是本计划所需的人员。 Cifuni 先生还接受过特殊动物手术方面的培训，例如眼眶后放血；尾随小鼠 基因分型；尾部 DNA 的 PCR 和 Southern 分析；固定组织用于组织学；尾静脉注射；等等。他是 能够教授该计划的其他成员这些程序