Sequencing the Human Microbiome

人类微生物组测序

• 批准号: 7847463
• 负责人: GEORGE M WEINSTOCK
• 金额: $ 428.23万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-22 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7847463
• 关键词: Bacteria; Cataloging; Catalogs; Censuses; Collection; Communities; DNA Sequence; Data; Data Set; Databases; Dermal; Disease; Gene Pool; Genes; Genome; Goals; Human; Human Microbiome; Human body; Individual; Knowledge; Maps; Metabolic; Metagenomics; Methods; Microbe; Nose; Oral; Organism; Pattern; Phenotype; Production; Reading; Ribosomal RNA; Sampling; Shotgun Sequencing; Shotguns; Site; Specimen; Structure; Vagina; Variant; Viral; Virus; Work; genome sequencing; member; metagenomic sequencing; microbial; microbiome; microorganism; public health relevance; rRNA Genes; tool; virome

DESCRIPTION (provided by applicant): The human microbiome is the collection of microorganisms that colonize the human body. Over the last year the analysis of the microbiome of 250 healthy subjects was initiated, focusing on five body sites (nasal, oral, vaginal, dermal, and gut) and many subsites. This study began to build a catalog of genome sequences of culturable bacteria that inhabit the human body as well as described the community structure formed by microbial species, allowing diversity to be assessed between body sites and between individuals. The goal of this study is to extend these initial probes in multiple directions. The study will add 1,000 genomes to the catalog of bacterial reference sequences of the human microbiome by using automated methods to isolate organisms and high throughput DNA sequencing to construct genome sequences. The study will also further characterize the metagenomes of the 250 individuals by directly comparing DNA sequences from organisms in the microbiome, thus deterimining what is common or variant between subjects. Going beyond bacteria, the project will characterize the virome, the component of the human microbiome formed by viruses, and take a census of eukaryotic microbes in the human microbiome. The last goal is to analyze the transcriptional patterns of microbes found in metagenomic communities, and by this means determine what functions are expressed in the different body niches. These studies will advance our growing knowledge of the human microbiome in healthy subjects, providing a baseline for disease studies as well as new tools for probing the microbiome. Public Health Relevance: The human microbiome is the collection of microorganisms that colonize the human body. Over the last year the analysis of the microbiome of 250 healthy subjects was initiated, focusing on five body sites. This study began to build a catalog of genome sequences of organisms that inhabit the human body as well as described the diversity found between body sites and individuals. The goal of this study is to add 1,000 genomes to the catalog of reference sequences of the human microbiome, to further characterize differences in the metagenomes of 250 individuals by directly comparing shotgun sequences, to characterize the virome component of the human microbiome, to take a census of eukaryotic microbes in the human microbiome, and to analyze the transcriptional pattern found in metagenomic communities. These studies will advance our growing knowledge of the human microbiome in healthy subjects, providing a baseline for disease studies as well as new tools for probing the microbiome.

描述（由申请人提供）：人类微生物组是定植于人体的微生物的集合。去年，我们开始对 250 名健康受试者的微生物组进行分析，重点关注五个身体部位（鼻腔、口腔、阴道、皮肤和肠道）和许多子部位。这项研究开始建立居住在人体中的可培养细菌的基因组序列目录，并描述微生物物种形成的群落结构，从而可以评估身体部位之间和个体之间的多样性。这项研究的目标是将这些初始探测扩展到多个方向。该研究将通过使用自动化方法分离生物体和高通量DNA测序来构建基因组序列，将1000个基因组添加到人类微生物组的细菌参考序列目录中。该研究还将通过直接比较微生物组中生物体的 DNA 序列来进一步表征 250 名个体的宏基因组，从而确定受试者之间的共同点或变异点。除了细菌之外，该项目还将描述病毒组（由病毒形成的人类微生物组的组成部分）的特征，并对人类微生物组中的真核微生物进行普查。最后一个目标是分析宏基因组群落中发现的微生物的转录模式，并通过这种方式确定不同的身体生态位表达哪些功能。这些研究将增进我们对健康受试者中人类微生物组的了解，为疾病研究提供基线以及探索微生物组的新工具。 公共卫生相关性：人类微生物组是寄生在人体中的微生物的集合。去年，我们开始对 250 名健康受试者的微生物组进行分析，重点关注五个身体部位。这项研究开始建立居住在人体中的生物体的基因组序列目录，并描述了身体部位和个体之间发现的多样性。本研究的目标是将1000个基因组添加到人类微生物组参考序列目录中，通过直接比较鸟枪法序列进一步表征250个个体宏基因组的差异，表征人类微生物组的病毒组成分，对人类微生物组中的真核微生物进行普查，并分析宏基因组群落中发现的转录模式。这些研究将增进我们对健康受试者中人类微生物组的了解，为疾病研究提供基线以及探索微生物组的新工具。