Dual release of osteogenic factors to enhance bone regeneration

双重释放成骨因子，增强骨再生

• 批准号: 7740042
• 负责人: Ambalangodage Champa Jayasuriya
• 金额: $ 11.24万
• 依托单位: UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-17 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7740042
• 关键词: Autologous; Biocompatible Materials; Biological; Biopolymers; Bone Marrow; Bone Regeneration; Bone Tissue; Cell Differentiation process; Cells; Chitosan; Clinical; Controlled Study; Cues; Culture Media; Data; Defect; Development; Encapsulated; Environment; Extracellular Matrix; Fracture; Growth Factor; Harvest; Healed; Hybrids; In Vitro; Injectable; Insulin-Like Growth Factor I; Kinetics; Methods; Mus; Natural regeneration; Osteoblasts; Osteogenesis; Phenotype; Principal Investigator; Public Health; Research; Stem cells; Stromal Cells; Testing; Tissues; Trauma; United States; base; bone; bone morphogenetic protein 2; calcium phosphate; controlled release; craniofacial; crosslink; healing; innovation; interest; novel; novel strategies; osteogenic; osteoprogenitor cell; public health relevance; reconstruction; repaired; scaffold

DESCRIPTION (provided by applicant): Reconstruction of craniofacial defects caused by fracture, trauma or degeneration is one of the major clinical challenges in the Unites States and around the world. The existing treatments have limitations and lack clinical predictability, necessitating the development of new strategies to heal, repair and regenerate the craniofacial bone tissues. The ability to regenerate new bone for craniofacial use is a significant clinical need. One approach to regenerate the tissues of interest is the seeding of autologous stem cells into a biomaterial or scaffold. These biomaterials or scaffolds support and provide cues for the cells, allowing them to grow, differentiate and secrete new extracellular matrix. It is necessary to develop new biodegradable materials, which provide an appropriate microenvironment for cell-matrix interactions that can mimic the important multi-functionality of the biological environment. This proposal focuses on development of a new approach for regenerating natural bone tissues, with emphasis on developing injectable hybrid microparticles (MPs) providing osteoconductivity, osteoinductivity and structural integrity into MPs. This will be achieved by following three specific research aims, as described below: 1. To fabricate and characterize hybrid MPs. 2. To optimize the in vitro release kinetics of growth factors. 3. To test and evaluate hybrid MPs for enhancement of osteogenesis in vitro using bone marrow stromal cells. Public Health Relevance: The relevance of this research to public health exists in the development of novel injectable hybrid microparticles that can be applied for regeneration of lost or damaged bone tissues.

描述（由申请人提供）：由骨折，创伤或变性引起的颅面缺陷的重建是联合国和世界各地的主要临床挑战之一。现有治疗方法有局限性，缺乏临床可预测性，因此需要制定新的策略来治愈，修复和再生颅面骨组织。再生新骨以颅面使用的能力是巨大的临床需求。再生感兴趣的组织的一种方法是将自体干细胞的播种到生物材料或支架中。这些生物材料或脚手架支持细胞的线索，从而使它们得以生长，区分和分泌新的细胞外基质。有必要开发新的可生物降解材料，该材料为细胞 - 矩阵相互作用提供适当的微环境，以模仿生物学环境的重要多功能性。该提案的重点是开发一种新方法来再生天然骨组织，重点是开发可注射的杂种微粒（MPS），从而为MPS提供了骨电导率，骨诱导和结构完整性。如下所述，将通过以下三个特定的研究目的来实现这一点：1。制造和表征混合MPS。 2。优化生长因子的体外释放动力学。 3。测试和评估杂种MPS使用骨髓基质细胞在体外增强体外的成骨。 公共卫生相关性：这项研究与公共卫生的相关性存在于可用于损失或受损的骨组织的新型混合微粒的开发中。