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CLINICAL CORE

临床核心

基本信息

批准号：
7933987
负责人：
HARVEY Ira PASS
金额：
$ 11.43万
依托单位：
UNIVERSITY OF HAWAII AT MANOA
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7933987
关键词：
Adult Air Aliquot Antibiotics Antibodies Asbestos Back Beds Benign Binding Biocompatible Materials Biological Preservation Biopsy Biopsy Specimen Blast Cell Buffers Carbon Carbon Dioxide Cell Culture Techniques Cell Fraction Cell Line Cells Centrifugation Chest Tubes Chloroform Chromosomes, Human, Pair 8 Clinical Clinical Data Collection Congestive Heart Failure Core Biopsy Country Culture Media DNA DNA Sequence Data Date of birth Decontamination Detection Diagnosis Digestion Disease Drug or chemical Tissue Distribution Dry Ice Electron Microscope Electrons Endopeptidase K Engineering Epidemiologic Studies Equipment Ethanol Event Excision Fiber Film Freezing Fresh Tissue Gel Gender Generations Genes Genome Genomics Gold Growth Hamsters Hand Hematoxylin and Eosin Staining Method Homidium Bromide Hospitals Hour Housing Human Human Genome Individual Informed Consent Institutes Investigation Laboratories Liquid substance Lung Lung nodule Malignant - descriptor Malignant Mesothelial Cell Malignant Neoplasms Malignant mesothelioma Malignant neoplasm of lung Mesothelial Cell Mesothelioma Mesothelium Methodology Microscopic Mineral Fibers Minority Molecular Molecular Weight Morphology Mutate Nature Needles Nitrogen Non-Malignant Normal tissue morphology Nucleic Acid Regulatory Sequences Nucleic Acids Operating Rooms Operative Surgical Procedures Oxygen PO-1 Paraffin Paraffin Embedding Pathogenesis Pathologic Pathology Patients Performance Peritoneum Phenols Plasma Plasmid Cloning Vector Plasmids Play Pleura Pleural Pleural effusion disorder Polyomavirus Population Potassium Hydroxide Procedures RNA Race Reaction Reading Reagent Recommendation Recording of previous events Recovery Replication Origin Research Research Personnel Resources Risk Role Sampling Sepharose Serum Services Shipping Ships Shoes Side Simian virus 40 Site Slide Source Southern Blotting Specimen Staging Staining method Stains Structure of parenchyma of lung Surface Techniques Testing Thoracic Oncology Thoracotomy Time Tissue Banks Tissue Microarray Tissue Sample Tissues Transmission Electron Microscopy Trypsin Tube Tumor Cell Line Tumor Tissue Ultraviolet Rays Validation Variant Veterans Hospitals Vial device Viral Virus Water Weight Work abstracting ammonium acetate anticancer research aqueous base beta Globin calretinin cancer care cancer cell carcinogenesis cohort design effusion feeding flasks isopentyl alcohol neoplastic cell outreach particle planetary Atmosphere prevent programs research facility response senescence square foot tissue culture tumor tumorigenesis

项目摘要

The Clinical Core housed at the Karmanos Cancer Institute is intended to be a resource for the program project as a whole and as a valuable source of biological materials necessary for the completion of the individual projects. The Clinical Core will be responsible for the collection, storage, and distribution of mesothelial cells and tissues, and mesothelioma tumors. The functions of the Clinical Core will be to serve as a resource for mesothelioma tumor tissue both snap frozen and paraffin embedded, and as a resource for asbestos-exposed pleura, lung, or peritoneum which is either snap frozen or paraffin embedded. The CORE will also serve as a resource for already established mesothelioma cell lines as well as an ongoing source of cultured human mesothelial cells from patients at the Karmanos Cancer Institute. New mesothelioma cell lines will be started prospectively from mesothleioma patients having resection. The Core will be responsible for the determining the SV40 status on these human mesothelial tissues, mesothelial cells, mesothelioma tumors and cultured mesothelioma cell lines in order to provide the appropriate reagents to the investigators. Tissue arrays for immunohistochemical validation of data will be constructed from paraffin embedded tumors and provided to the individual investigators. The Clinical Core will also coordinate the performance of asbestos lung content analysis from patients with mesothelioma for correlative data in the individual projects. It is estimated that 60-80 new mesothelioma cases per year will be seen at the Karmanos Cancer Institute during the course of the program project, and it is conservatively estimated that 20-30 of these will be accrued as fresh/frozen specimens. Paraffin embedded tumors will be available from all cases. Tumor samples will be used in Projects 1 and 3, and cell lines/cultures will be used in projects 2 and 3. By centralizing these services into a Core, the collection, storage, analysis, and distribution of tissues relevant to mesothelioma carcinogenesis will be better managed and coordinated for the entire Program Project.

位于Karmanos癌症研究所的临床核心旨在成为该计划的资源项目作为一个整体，并作为一个有价值的生物材料来源，个别项目。临床中心将负责收集、储存和分发间皮瘤细胞和组织以及间皮瘤肿瘤。临床中心的职能是作为快速冷冻和石蜡包埋的间皮瘤肿瘤组织的资源，暴露于石棉的胸膜、肺或腹膜，快速冷冻或石蜡包埋。核心还将作为已经建立的间皮瘤细胞系的资源以及持续的来源 Karmanos癌症研究所的患者培养的人类间皮细胞。新间皮瘤细胞将从切除的间皮瘤患者前瞻性地开始。核心将负责用于确定这些人间皮瘤组织、间皮瘤细胞、间皮瘤肿瘤和培养的间皮瘤细胞系，以便为研究者提供适当的试剂。用于免疫组化数据验证的组织阵列将从石蜡包埋的肿瘤构建并提供给个体研究者。临床核心还将协调以下方面的绩效：间皮瘤患者肺石棉含量分析，以获得个体项目中的相关数据。据估计，60 - 80新的间皮瘤病例，每年将看到在Karmanos癌症研究所在计划项目的过程中，保守估计，其中20 - 30个将是作为新鲜/冷冻标本累积。石蜡包埋的肿瘤将从所有病例中获得。肿瘤样品将用于项目1和3，细胞系/培养物将用于项目2和3。通过将这些服务集中到一个核心中，收集、存储、分析和分发与间皮瘤癌变将得到更好的管理和协调整个计划项目。