La Jolla Interdisciplinary Neuroscience Center Cores - Structural Bio Core BU

拉荷亚跨学科神经科学中心核心 - 结构生物核心事业部

• 批准号: 7925706
• 负责人: STUART A LIPTON
• 金额: $ 8.9万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7925706
• 关键词: Amino Acids; Area; Arts; Binding; Binding Proteins; Bromides; Cancer Center; Carbohydrate Chemistry; Cells; Charge; Chemistry; Collaborations; Collection; Complex; Computer software; Consultations; Coupling; Crystallization; Crystallography; Data; Data Collection; Data Set; Databases; Faculty; Fostering; Funding; Future; Goals; Grant; Head; Homologous Gene; Housing; Informatics; Institutes; Investments; Journals; Label; Laboratories; Learning; Mainstreaming; Measurement; Methodology; Methods; Molecular Structure; NMR Spectroscopy; NOESY; Nature; Neurosciences; Nuclear Magnetic Resonance; Organic Synthesis; Paper; Peptides; Phase; Plant Resins; Postdoctoral Fellow; Preparation; Proteins; Relaxation; Research Personnel; Research Project Grants; Residual state; Resolution; Roentgen Rays; Route; Sampling; Science; Selenomethionine; Services; Set protein; Side; Solutions; Solvents; Source; Staging; Structural Biologist; Structure; Synchrotrons; System; Techniques; Testing; Training; Vertebral column; Wisconsin; Work; X ray diffraction analysis; X-Ray Crystallography; X-Ray Diffraction; base; combinatorial; data management; design; drug discovery; high throughput screening; improved; inhibitor/antagonist; instrumentation; interest; member; protein expression; protein structure; research study; small molecule; small molecule libraries; solid state nuclear magnetic resonance; structural biology; three dimensional structure

STRUCTURAL BIOLOGY CORE 1 . MAIN OBJECTIVES AND NEW DIRECTIONS The use of Structural Biology on the La Jolla Torrey Pines Mesa has increased tremendously over the past 5 years. For example, BIMR has added 4 new faculty members as major users of its Structural Biology Facility and is directed by crystallography Robert Liddington. However, presently this Core is part of the Burnham NIH-funded Cancer Center with little or no access to neuroscientists on the La Jolla Torrey Pines Mesa. Additionally, two faculty at The Salk are also heavily invested in crystallography but heretofore were not organized as a Core and thus have little interaction with neuroscientists. Moreover, the Head of NMR at UCSD, Stan Opella, is world-renowned in this area and has an NMR facility that would be made available to neuroscientists on this grant as a satellite core. Hence, the structural core will function with headquarters at BIMR and satellite facilities at The Salk Institute and UCSD. This Structural Core will foster collaborative interactions by allowing equal access to neuroscientists. BIMR has made major investments in instrumentation for structural biology, including the acquisition of three new NMR spectrometers and upgrades for the X-ray diffraction set up. UCSD has installed a state-or-the-art 900 MHz spectrometer, which will be available for collaborations of neuroscientists. Scientific progress has been impressive in the recent past by these facilities, including 6 papers in the journals Cell, Science and Nature that utilized structural data from the facilities. However, Neuroscientists have had minimal, if any, access to the faculty or instrumentation because they are located within the Cancer Centers at BIMR, TSRI, UCSD, and The Salk. The current grant will rectify this by making the facility and the faculty available to Neuroscientists as a Core Unit. In summary, the Structural Biology Core comprises two facilities: Crystallography and NMR. Scientific, logistical, and budgetary issues provide justification for this merger of services. The primary application of Crystallography and NMR overlap, and most research projects require both Crystallography and NMR. The merged facility serves the needs of users with a broad range of expertise. There are several neuroscience investigators who are not mainstream structural biologists but who have a desire to learn structural techniques. The facility provides appropriate training and direction for these people. Other "hardcore" structural groups require much less support, and the facility will primarily supply the necessary hardware and technical support to achieve the scientific goals. One manager (who is free to this grant) oversees both techniques within the Structural Biology Facility and provides the necessary training (in conjunction with the faculty supervisors); technicians paid for by the current grant will coordinate the efforts at the three centers (BIMR, Salk, UCSD, and facilitate TSRI neuroscientist access as well). Future plans include a protein expression facility in this Core.

结构生物学核心 1 .主要举措和新方向 结构生物学在拉霍亚托里松树梅萨上的应用已经大大增加， 了过去5年例如，BIMR增加了4名新的教员，作为其 结构生物学设施，由晶体学罗伯特Liddington指导。但是当前 这个核心是伯纳姆国家卫生研究院资助的癌症中心的一部分，很少或根本没有神经科学家 在拉霍亚托里派恩斯梅萨。此外，索尔克的两名教师也投入巨资， 晶体学，但迄今为止没有组织成核心，因此与 神经科学家此外，UCSD的NMR负责人Stan Opella在该领域享有世界声誉 并且有一个核磁共振设备，可以作为卫星提供给神经科学家使用 核心因此，结构核心将与设在BIMR的总部和设在 索尔克研究所和加州大学圣地亚哥分校。这一结构核心将通过允许平等的 接触神经科学家BIMR在结构生物学仪器方面进行了大量投资， 包括购置三台新的核磁共振光谱仪和升级X射线衍射仪 起来加州大学圣地亚哥分校已经安装了一个国家或最先进的900兆赫频谱仪，这将是可用于 神经科学家的合作。科学进步在最近的过去令人印象深刻， 设施，包括6篇论文在杂志细胞，科学和自然，利用结构数据，从 设施。然而，神经科学家们几乎没有机会接触到教师， 仪器，因为它们位于BIMR，TSRI，UCSD和The 索尔克目前的赠款将纠正这一点，使设施和教师提供给 神经科学家作为核心单位。 总之，结构生物学核心包括两个设施：晶体学和NMR。 科学、后勤和预算问题为这种服务合并提供了理由。主 晶体学和核磁共振的应用重叠，大多数研究项目都需要两者 晶体学和NMR。合并后的设施可满足用户的各种需求， 专业知识有几个神经科学研究者不是主流的结构生物学家 但他们渴望学习结构技术。该设施提供适当的培训， 这些人的方向。其他“核心”结构组需要的支持要少得多， 该设施将主要提供必要的硬件和技术支持，以实现科学的 目标.一个经理（谁是免费的，这个赠款）监督结构生物学内的两种技术 设施和提供必要的培训（与教师主管一起）;技术人员 由当前拨款支付的资金将协调三个中心（BIMR，Salk，UCSD和 也便于TSRI神经科学家访问）。未来的计划包括在这一领域建立一个蛋白质表达设施。 核心