MS FOR PROBING RESISTANCE STRATEGIES IN ACETIC ACID BACTERIA

用于探测乙酸细菌耐药策略的 MS

• 批准号: 7953999
• 负责人: THOMAS JOSEPH KAPPOCK
• 金额: $ 0.47万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953999
• 关键词: Acetic Acids; Acetobacter; Acids; Bacteria; Biochemical; Cell membrane; Cellular Structures; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Drops; Environment; Enzymes; Funding; Grant; Individual; Institution; Life; Mass Spectrum Analysis; Metabolic; Microbe; Organism; Plants; Production; Proteins; Research; Research Personnel; Resistance; Resources; Source; United States National Institutes of Health; Vinegar; X-Ray Crystallography; bacterial resistance; biological adaptation to stress; biomedical resource; coping; dimer; genome sequencing; pathogen; resistant strain

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bacteria cope with life in harsh environments using specific stress responses and chemical alterations of cell components. We explore the biochemical strategies for survival at low pH employed by a naturally acid-resistant bacterium, Acetobacter aceti, which has the ability to handle low pH in general -- its cytoplasmic pH can drop as low as 4 -- and acetic acid in particular. This Gram-negative, plant-associated bacterium has been used for millenia in the production of vinegar. Acetic acid is especially toxic to bacteria at low pH because it forms dimers that easily permeate cell membranes. Kappock's approach to this problem is centered on enzyme function at low pH, assisted by several in-progress genome sequencing projects. We found that individual A. aceti enzymes retain function in acid much longer than comparable forms from non-acidophilic organisms. X-ray crystallography has revealed distinctive architectural features of A. aceti proteins that may confer this stability. We have also delineated metabolic strategies for acetic acid elimination by highly-resistant strains, which may be employed by other microbes including several pathogens.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 细菌利用特定的应激反应和细胞成分的化学变化来科普恶劣环境中的生命。我们探索了在低pH值下生存的生化策略，该策略由天然耐酸细菌Acetobacter aceti采用，该细菌具有处理低pH值的能力-其细胞质pH值可以下降至4 -特别是乙酸。这种革兰氏阴性，植物相关的细菌已被用于生产醋千年。乙酸在低pH下对细菌尤其有毒，因为它形成容易渗透细胞膜的二聚体。 Kappock对这个问题的研究主要集中在酶在低pH值下的功能上，并得到了几个正在进行的基因组测序项目的帮助。我们发现A.乙酰酶在酸中保持功能的时间比来自非嗜酸生物的可比形式长得多。X射线晶体学揭示了A.乙酰化的蛋白质可以赋予这种稳定性。我们还描述了高耐药菌株消除乙酸的代谢策略，这些策略可能被其他微生物包括几种病原体所采用。