Systems Biology Core

系统生物学核心（核心B）的总体目标是提供经验，培训和设施， 高通量测量，以及生物信息学和系统分析方面的专业知识， 帮助PPG的项目研究人员在系统级上生成和测试新的假设 短期和长期缺氧耐受性和易感性的机制。具体而言，核心B将提供 （a）获取和分析人类、小鼠和果蝇基因组的高通量数据， 在缺氧环境中的表达和遗传序列变异以及感兴趣的信号传导途径; （B）通过NMR获取和分析在正常和缺氧条件下的代谢谱和活性 代谢组学、代谢网络重建和计算建模;（c）执行特定的 在苍蝇和老鼠的表型测量和开发生理动力学的计算模型; 以及（d）部署和管理生理数据库、网站和网络工具， 传播数据和调查结果。核心B将带来微阵列和通路分析方面的专业知识， 代谢生物化学和代谢组学，生物工程和系统生物学与所有三个工作 项目和核心C在各种测量和数据分析。另外。核心B将负责 用于基于网络部署和维护计划项目的数据传播资源。

The overall goal of the Systems Biology Core (Core B) is to provide experience, training and facilities for high-throughput measurements, together with expertise in bioinformatics and systems analysis in order to help the project investigators of the PPG to generate and test new hypotheses on systems-level mechanisms of short- and long-term hypoxia tolerance and susceptibility. Specifically, Core B will provide expertise and resources to: (a) acquire and analyze high-throughput data in humans, mice and flies on gene expression and genetic sequence variations in the context of hypoxia and the signaling pathways of interest; (b)acquire and analyze metabolic profiles and activity under normal and hypoxic conditions by NMR metabolomics, metabolic network reconstruction, and computational modeling; (c) to perform specific phenotypic measurements in flies and mice and develop computational models of physiological dynamics; and (d) deployment and curation of a physiological database and a web site and web-based tools for timely dissemination of data and findings. Core B will bring expertise in microarrays and pathway analysis, metabolic biochemistry and metabolomics, bioengineering and systems biology to work with all three projects and Core C in a variety of measurements and data analysis. In addition. Core B will be responsible for the web-based deployment and maintenance of data dissemination resources for the program project.