Biosynthesis of Indolic Phytoalexins: Mechanisms of Plant Innate Immune Response

吲哚植物抗毒素的生物合成：植物先天免疫反应的机制

基本信息

批准号：
7772503
负责人：
Elizabeth Susan Sattely
金额：
$ 9万
依托单位：
HARVARD MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-01-11 至 2010-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7772503
关键词：
Acetonitriles Anabolism Antiparasitic Agents Arabidopsis Area Auxins Biochemistry Biogenesis Bioinformatics Biological Assay Biological Factors Broccoli - dietary Carbon Chemicals Chemistry Collaborations Complex Consult Cysteine Cytochrome P450 Data Development Drug Formulations Ecology Educational workshop Enzymatic Biochemistry Enzymes Foundations Genes Genetic Glucosinolates Health Hormones Host Defense Human Immune response Indoles Investigation Kale - dietary Ligation Medicine Mentors Metabolism Methods Mixed Function Oxygenases Molecular Orphan Pathway interactions Phase Plant Components Plant Model Plants Prevalence Production Property Recombinants Research Research Proposals Role Route Stress System Training Vegetables Work antimicrobial biological adaptation to stress defense response enzyme pathway interdisciplinary approach meetings member metabolomics pathogen phytoalexin phytoalexins programs public health relevance skills small molecule ward

项目摘要

DESCRIPTION (provided by applicant): This research proposal describes the development of a research program to study the biogenesis of Arabidopsis phytoalexins. We will begin by investigating the biosynthetic pathway of camalexin, a phytoalexin produced as part of the induced defense response. The first part of our two-phase plan for studying Arabidopsis phytoalexin pathways will focus on the identification of camalexin biosynthetic genes through a collaboration with the Ausubel lab at MGH. During this training period, I will attend relevant meetings and workshops, and I will consult regularly with three senior advisors to establish skills required for timely advance of the project. During the second, independent phase of the project I will biochemically characterize candidate phytoalexin pathway enzymes implicated by the data gathered during phase one. I expect that this work will serve as a springboard for the formulation of a new research program that uses a combination of genetics, chemistry, and biochemistry to elucidate secondary metabolite biosynthetic pathways in plants. A deeper understanding of these pathways will not only illuminate the molecular details of host-pathogen interactions, but it is also likely to have an important impact on human health, given the strong track record of plant natural products as clinically-used medicines. Public Health Relevance: Plants produce a wealth of secondary metabolites for defense against destructive pests. The research program outlined in this proposal explores components of plant immune response pathways as well as the production, distribution, and metabolism of the contributing small molecules. These compounds are contained in commonly consumed vegetables and have been found to have beneficial effects on human health.

描述（由申请人提供）：该研究提案描述了研究拟南芥植物毒素生物发生的研究计划的发展。我们将首先研究Camalexin的生物合成途径，Camalexin是一种作为诱导防御反应的一部分产生的植物甲状腺素。我们研究拟南芥植物毒素途径的两相计划的第一部分将通过与MGH的Ausubel Lab合作，重点介绍Camalexin Biosynthetic Genes的鉴定。在此培训期间，我将参加相关的会议和研讨会，并将定期与三位高级顾问咨询，以确定及时提前该项目所需的技能。在该项目的第二个独立阶段，我将在生物化学上表征候选植物抗毒素途径的酶，该酶与第一阶段期间收集的数据有关。我希望这项工作将成为制定新研究计划的跳板，该计划结合了遗传学，化学和生物化学来阐明植物中的二级代谢物生物合成途径。对这些途径的更深入的了解不仅会阐明宿主 - 病原体相互作用的分子细节，而且考虑到植物天然产物作为临床中使用的药物的良好记录，它也可能对人类健康产生重要影响。公共卫生相关性：植物产生大量的二级代谢产物，以防御破坏性害虫。该提案中概述的研究计划探讨了植物免疫反应途径的组成部分，以及促成小分子的生产，分布和代谢。这些化合物包含在普遍消耗的蔬菜中，并被发现对人类健康产生有益的影响。