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Biosynthesis of Indolic Phytoalexins: Mechanisms of Plant Innate Immune Response

吲哚植物抗毒素的生物合成：植物先天免疫反应的机制

基本信息

批准号：
8425108
负责人：
Elizabeth Susan Sattely
金额：
$ 24.02万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-01-11 至 2015-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8425108
关键词：
Acetonitriles Anabolism Antiparasitic Agents Arabidopsis Area Auxins Biochemistry Biogenesis Bioinformatics Biological Assay Biological Factors Broccoli - dietary Carbon Chemicals Chemistry Collaborations Complex Consult Cysteine Cytochrome P450 Data Development Drug Formulations Ecology Educational workshop Enzymatic Biochemistry Enzymes Foundations Genes Genetic Glucosinolates Health Hormones Host Defense Human Immune response Indoles Investigation Ligation Medicine Mentors Metabolism Methods Mixed Function Oxygenases Molecular Orphan Pathway interactions Phase Plant Components Plant Model Plants Prevalence Production Property Recombinants Research Research Proposals Role Route System Training Vegetables Work antimicrobial biological adaptation to stress defense response enzyme pathway interdisciplinary approach meetings member metabolomics pathogen phytoalexin phytoalexins programs skills small molecule ward

项目摘要

¿ Project Summary This research proposal describes the development of a research program to study the biogenesis of Arabidopsis phytoalexins. We will begin by investigating the biosynthetic pathway of camalexin, a phytoalexin produced as part of the induced defense response. The first part of our two-phase plan for studying Arabidopsis phytoalexin pathways will focus on the identification of camalexin biosynthetic genes through a collaboration with the Ausubel lab at MGH. During this training period, I will attend relevant meetings and workshops, and I will consult regularly with three senior advisors to establish skills required for timely advance of the project. During the second, independent phase of the project I will biochemically characterize candidate phytoalexin pathway enzymes implicated by the data gathered during phase one. I expect that this work will serve as a springboard for the formulation of a new research program that uses a combination of genetics, chemistry, and biochemistry to elucidate secondary metabolite biosynthetic pathways in plants. A deeper understanding of these pathways will not only illuminate the molecular details of host-pathogen interactions, but it is also likely to have an important impact on human health, given the strong track record of plant natural products as clinically-used medicines.

项目概要本研究提案描述了一项研究计划的发展，拟南芥植物抗毒素的生物发生。我们将开始通过研究生物合成 Camalexin是一种作为诱导防御反应的一部分产生的植物抗毒素。我们研究拟南芥植物抗毒素途径的两阶段计划的第一部分将重点是通过与麻省总医院的奥苏贝尔实验室在培训期间，我将参加有关会议，我将定期与三位高级顾问协商，及时推进项目所需。在第二个独立阶段，项目I将对候选植物抗毒素途径酶进行生物化学表征在第一阶段收集的数据中发现的我希望这项工作将作为一个一个新的研究计划，使用的组合跳板遗传学、化学和生物化学，以阐明次级代谢物的生物合成植物的路径对这些途径的更深入了解不仅会阐明宿主-病原体相互作用的分子细节，但它也可能有一个对人类健康的重要影响，鉴于植物天然产品作为临床用药。