Biosynthesis of Indolic Phytoalexins: Mechanisms of Plant Innate Immune Response

？项目摘要 这项研究计划描述了一项研究计划的发展，以研究 拟南芥植物保卫素的生物发生。我们将从研究生物合成的 Camalexin途径，作为诱导防御反应的一部分产生的一种植物保护蛋白。 我们研究拟南芥植物保卫素途径的两个阶段计划的第一部分将 通过与合作伙伴的合作，专注于鉴定Camalexin生物合成基因 麻省理工学院的奥苏贝尔实验室。在此培训期间，我将参加相关会议并 研讨会，我将定期咨询三名高级顾问，以建立技能 为及时推进项目所需。在第二个独立的阶段， 项目I将对候选植物保卫素途径酶进行生化表征 与第一阶段收集的数据有关。我希望这项工作将成为一种 制定一项新的研究计划的跳板，该计划结合使用 遗传学、化学和生物化学阐明次生代谢物生物合成 植物中的路径。对这些途径的更深入的了解不仅将有助于 宿主-病原体相互作用的分子细节，但它也可能有一个 对人类健康的重要影响，考虑到植物天然的良好记录 作为临床用药的产品。

¿ Project Summary This research proposal describes the development of a research program to study the biogenesis of Arabidopsis phytoalexins. We will begin by investigating the biosynthetic pathway of camalexin, a phytoalexin produced as part of the induced defense response. The first part of our two-phase plan for studying Arabidopsis phytoalexin pathways will focus on the identification of camalexin biosynthetic genes through a collaboration with the Ausubel lab at MGH. During this training period, I will attend relevant meetings and workshops, and I will consult regularly with three senior advisors to establish skills required for timely advance of the project. During the second, independent phase of the project I will biochemically characterize candidate phytoalexin pathway enzymes implicated by the data gathered during phase one. I expect that this work will serve as a springboard for the formulation of a new research program that uses a combination of genetics, chemistry, and biochemistry to elucidate secondary metabolite biosynthetic pathways in plants. A deeper understanding of these pathways will not only illuminate the molecular details of host-pathogen interactions, but it is also likely to have an important impact on human health, given the strong track record of plant natural products as clinically-used medicines.