Physiological effects of hyperosmotic vocal fold surface fluid

高渗声带表面液体的生理效应

• 批准号: 7714355
• 负责人: Preeti M. Sivasankar
• 金额: $ 14.66万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2011-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7714355
• 关键词: Address; Affect; Air; Allergens; Biological; Biomechanics; Breathing; Cell physiology; Chemicals; Chloride Ion; Chlorides; Clinical; Data; Dehydration; Economics; Environment; Epithelial; Exposure to; Family suidae; Fatigue; Goals; Health; Hour; Human; Humidity; Hydration status; In Vitro; Individual; Inflammation Mediators; Investigation; Ion Transport; Liquid substance; Measures; Mouth Breathing; Mucous Membrane; Oral; Osmolar Concentration; Participant; Particulate; Permeability; Persons; Phonation; Physiological; Physiology; Prevention; Production; Property; Proteins; Protocols documentation; Reflux; Relative (related person); Reporting; Research; Resistance; Role; Salts; Smoke; Sodium; Stress; Surface; Tissues; Voice; base; comparison group; cost; human subject; improved; in vivo; lung pressure; novel; particle; particulate pollutant; pathogen; pollutant; pressure; prevent; research study; vocal cord

DESCRIPTION (provided by applicant): The goal of this application is to measure the effects of hyperosmotic fluid environment on vocal fold mucosal physiology and vocal function. Previous research has suggested that the isosmotic fluid environment of the vocal folds is necessary for regulating vocal fold mucosal physiology and ease of voice production. However, the isosmotic fluid environment of the vocal folds is easily perturbed by everyday exposure to poorly conditioned inhaled air, smoke, and pollutants. These perturbations raise the concentration of salts, proteins, and particulate in the effects of hyperosmotic vocal fold surface fluid on vocal function and mucosal physiology surface fluid, creating a hyperosmotic environment. Data from other biological tissues indicate that a hyperosmotic surface environment is detrimental to tissue physiology, but, the effects of hyperosmotic vocal fold surface fluid (VFSF) on vocal fold mucosal physiology and vocal function are not known. This application will utilize a combination of native tissue protocols and human subject experiments to (1) Measure the effects of hyperosmotic surface fluid on vocal fold mucosal ion transport, and production of inflammatory mediators (2) Characterize the chemical composition of hyperosmotic VFSF in healthy subjects and speakers reporting vocal fatigue, who are completing an oral breathing challenge at low humidity (3) Examine whether the physiological effects of hyperosmotic VFSF induced by oral breathing at low humidity are evident in clinical measures of Phonation Threshold Pressure and (4) Demonstrate reversal of the effects of hyperosmotic VFSF induced during oral breathing, by humidifying inhaled air. These translational experiments will address the relevance of studying the effects of hyperosmotic surface fluid on vocal fold mucosal physiology by demonstrating that hyperosmotic VFSF persists in speakers with vocal fatigue. Further, this application will offer novel empirical evidence for the beneficial effects of humidifying ambient air in restoring the local fluid environment of the vocal folds. Improved understanding of the chemical composition of VFSF, and the role of VFSF in regulating mucosal physiology, are paramount to developing scientifically-grounded hydration treatments, and personalized pharmacological strategies for individuals with voice problems. Hyperosmotic vocal fold surface fluid results from everyday challenges to the local vocal fold environment. This application will demonstrate that hyperosmotic vocal fold surface fluid adversely affects mucosal physiology and is a proinflammatory stress, but that these detrimental effects can be prevented by to examine the interdependencies among the dependent variables. Given the enormous economic and societal costs associated with treating voice problems, the focus on prevention is both timely and relevant.

描述（由申请人提供）：本申请的目的是测量高渗液环境对声带粘膜生理学和发声功能的影响。先前的研究表明，声带的等渗流体环境对于调节声带粘膜生理和易于发声是必要的。然而，声带的等渗流体环境很容易因每天接触条件较差的吸入空气、烟雾和污染物而受到干扰。这些扰动提高了盐、蛋白质和颗粒的浓度，从而影响高渗声带表面液体对发声功能和粘膜生理学表面液体的影响，从而形成高渗环境。来自其他生物组织的数据表明高渗表面环境对组织生理学有害，但是高渗声带表面液（VFSF）对声带粘膜生理学和发声功能的影响尚不清楚。该应用将结合天然组织方案和人体实验来 (1) 测量高渗表面流体对声带粘膜离子转运和炎症介质产生的影响 (2) 表征在低湿度下完成口腔呼吸挑战的健康受试者和报告声音疲劳的说话者中高渗 VFSF 的化学成分 (3) 检查 低湿度下口腔呼吸引起的高渗性 VFSF 在发声阈值压力的临床测量中很明显，并且 (4) 通过加湿吸入的空气来证明口腔呼吸过程中引起的高渗性 VFSF 的影响的逆转。这些转化实验将通过证明高渗性 VFSF 在患有声音疲劳的说话者中持续存在，来解决研究高渗性表面液体对声带粘膜生理学影响的相关性。此外，该应用将为加湿环境空气在恢复声带局部流体环境方面的有益效果提供新颖的经验证据。更好地了解 VFSF 的化学成分以及 VFSF 在调节粘膜生理学中的作用，对于开发有科学依据的水合治疗方法以及针对有声音问题的个体的个性化药理学策略至关重要。高渗声带表面液体是由于局部声带环境的日常挑战造成的。该应用将证明高渗声带表面液体对粘膜生理学产生不利影响，并且是一种促炎应激，但可以通过检查因变量之间的相互依赖性来预防这些不利影响。鉴于治疗嗓音问题会带来巨大的经济和社会成本，因此对预防的关注既及时又有意义。