权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Physiological effects of hyperosmotic vocal fold surface fluid

高渗声带表面液体的生理效应

基本信息

批准号：
7991773
负责人：
Preeti M. Sivasankar
金额：
$ 14.21万
依托单位：
PURDUE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-12-01 至 2012-11-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The goal of this application is to measure the effects of hyperosmotic fluid environment on vocal fold mucosal physiology and vocal function. Previous research has suggested that the isosmotic fluid environment of the vocal folds is necessary for regulating vocal fold mucosal physiology and ease of voice production. However, the isosmotic fluid environment of the vocal folds is easily perturbed by everyday exposure to poorly conditioned inhaled air, smoke, and pollutants. These perturbations raise the concentration of salts, proteins, and particulate in the effects of hyperosmotic vocal fold surface fluid on vocal function and mucosal physiology surface fluid, creating a hyperosmotic environment. Data from other biological tissues indicate that a hyperosmotic surface environment is detrimental to tissue physiology, but, the effects of hyperosmotic vocal fold surface fluid (VFSF) on vocal fold mucosal physiology and vocal function are not known. This application will utilize a combination of native tissue protocols and human subject experiments to (1) Measure the effects of hyperosmotic surface fluid on vocal fold mucosal ion transport, and production of inflammatory mediators (2) Characterize the chemical composition of hyperosmotic VFSF in healthy subjects and speakers reporting vocal fatigue, who are completing an oral breathing challenge at low humidity (3) Examine whether the physiological effects of hyperosmotic VFSF induced by oral breathing at low humidity are evident in clinical measures of Phonation Threshold Pressure and (4) Demonstrate reversal of the effects of hyperosmotic VFSF induced during oral breathing, by humidifying inhaled air. These translational experiments will address the relevance of studying the effects of hyperosmotic surface fluid on vocal fold mucosal physiology by demonstrating that hyperosmotic VFSF persists in speakers with vocal fatigue. Further, this application will offer novel empirical evidence for the beneficial effects of humidifying ambient air in restoring the local fluid environment of the vocal folds. Improved understanding of the chemical composition of VFSF, and the role of VFSF in regulating mucosal physiology, are paramount to developing scientifically-grounded hydration treatments, and personalized pharmacological strategies for individuals with voice problems. Hyperosmotic vocal fold surface fluid results from everyday challenges to the local vocal fold environment. This application will demonstrate that hyperosmotic vocal fold surface fluid adversely affects mucosal physiology and is a proinflammatory stress, but that these detrimental effects can be prevented by to examine the interdependencies among the dependent variables. Given the enormous economic and societal costs associated with treating voice problems, the focus on prevention is both timely and relevant.

描述（由申请人提供）：本申请的目的是测量高渗液体环境对声带粘膜生理学和发声功能的影响。以往的研究表明，声带的等渗流体环境是必要的，以调节声带粘膜的生理和容易的声音产生。然而，声带的等渗流体环境很容易受到日常暴露于条件差的吸入空气、烟雾和污染物的干扰。这些扰动提高了盐、蛋白质和颗粒物的浓度，使高渗声带表面流体对发声功能和粘膜生理表面流体产生影响，从而产生高渗环境。来自其他生物组织的数据表明，高渗表面环境对组织生理有害，但是，高渗声带表面液（VFSF）对声带粘膜生理和发声功能的影响尚不清楚。本申请将利用天然组织方案和人类受试者实验的组合来（1）测量高渗表面流体对声带粘膜离子转运和炎症介质产生的影响（2）表征健康受试者和报告发声疲劳的说话者中的高渗VFSF的化学组成，在低湿度下完成口呼吸挑战的患者（3）检查在低湿度下口呼吸诱导的高渗VFSF的生理效应在发声阈值压力的临床测量中是否明显，以及（4）证明通过加湿吸入空气可以逆转经口呼吸期间诱导的高渗VFSF的影响。这些翻译实验将解决的相关性研究高渗表面流体对声带粘膜生理的影响，通过证明高渗VFSF持续在扬声器与声乐疲劳。此外，这一应用将提供新的经验证据的有益效果，呼吸环境空气在恢复局部流体环境的声带。对VFSF的化学组成以及VFSF在调节粘膜生理学中的作用的更好理解，对于开发基于科学的水合治疗和针对语音问题的个体的个性化药理学策略至关重要。高渗性声带表面液体是由于日常对局部声带环境的挑战。本申请将证明，高渗声带表面液对粘膜生理产生不利影响，是一种促炎性应激，但这些不利影响可以通过检查因变量之间的相互依赖关系来预防。考虑到治疗嗓音问题所需的巨大经济和社会成本，将重点放在预防上是及时和相关的。