Endocytic and Exocytic Traffic in Bladder Uroepithelium

项目主管：Apodaca，Gerard L. 项目叙事。排列在膀胱内表面的伞状细胞必须调整它们的膜 膀胱充盈和排空时的区域。面积的增加是通过插入(胞吐)一池 细胞表面下面的小泡，而面积的减少是通过内化细胞表面的 通过一种称为内吞作用的过程添加膜。理解胞吐和胞内吞噬是 重要的是因为细菌使用这些过程进入和离开膀胱细胞和缺陷 胞吐/胞吞作用可能是细胞表面蛋白质表达改变的原因，在 间质性膀胱炎等疾病。 项目主管：Apodaca，Gerard L. 设施： 实验室： 该项目将在位于斯凯夫9“^楼的肾脏电解液部门实验室进行 霍尔。Apodaca博士的Scaife实验室有600平方英尺的空间用于膀胱研究， 以及毗邻的一个400平方英尺的实验室，用于研究MDCK细胞。其他设施包括一个100平方英尺的 一个100平方英尺的房间，里面有我们的扫描电子显微镜，一个200平方英尺的细胞培养物 设施，位于大厅对面的一个400平方英尺的显微镜房间，以及相邻的设备室。 临床： 不适用 动物： 在新装修的动物保护设施的10层提供了小型和大型哺乳动物的住所 位于南生物医学科学塔，毗邻斯凯夫厅。 计算机： Apodaca实验室配备了几台PowerPC Macintoshes(G4和G5)、一台HP 4000系列激光器 打印机，Tektronix 840彩色激光打印机，柯达染料升华打印机，Agfa Arcus II桌面扫描仪， 以及几个文字处理、图形和图像分析软件包，包括Volocity。这个 实验室还配备了一台苹果生物信息学服务器，带有2TB的RAID存储设备。 办公室： 阿波达卡博士的办公室毗邻实验室，面积为200平方英尺。 其他： 机械和电子商店位于斯凯夫音乐厅的地下室。他们的服务包括设计、 设备的制造和维修。匹兹堡大学转基因和基因打靶设施和 蛋白质组学设施位于医学院邻近的生物科学塔内。

Program Director: Apodaca, Gerard L. Project Narrative. The umbrella cells that line the inner surface of the bladder must adjust their membrane area as the bladder fills and empties. Increases in area are mediated by insertion (exocytosis) of a pool of small vesicles that underlie the cell surface, while decreases in area are accomplished by internalizing the added membrane through a process called endocytosis. Understanding exocytosis and endocytosis is important because bacteria use these processes to enter and exit bladder cells and defects in exocytosis/endocytosis may account for the altered cell surface expression of proteins that is observed in diseases like interstitial cystitis. Program Director: Apodaca, Gerard L. Facilities: Laboratory: This project will be performed in the Renal-Electrolyte Division Laboratories, located on the 9"^ floor of Scaife Hall. Dr. Apodaca's Scaife laboratory includes 600 square feet of space that is devoted to bladder research, and an adjoining 400 square foot laboratory for work with MDCK cells. Additional facilities include a 100 square foot room that houses our TEM, a 100 square foot room that houses our SEM, a 200 square foot cell culture facility, a 400 square foot microscopy room located across the hall, and adjacent equipment rooms. Clinical: N/A Animal: Housing for small and large mammals is provided on the 10th floor of the newly renovated Animal Care facility located in the South Biomedical Science Tower, adjacent to Scaife Hall. Computer: The Apodaca laboratory is equipped with several PowerPC Macintoshes (G4 and G5), an HP 4000 series laser printer, a Tektronix 840 color laser printer, a Kodak dye sublimation printer, an Agfa Arcus II desktop scanner, and several word processing, graphics, and image analysis software packages including Volocity. The laboratory also houses an Apple Bioinformatics Server with a 2 terabyte RAID storage device. Office: Dr. Apodaca's office is adjacent to the laboratory and is 200 square feet in area. Other: Machine and electronic shops are located in the basement of Scaife Hall. Their services include design, fabrication, and repair of equipment. The University of Pittsburgh Transgenic and Gene Targeting Facility and the Proteomics Facility are located in the adjacent Bioscience Towers of the School of Medicine.