Resource Development Core

资源开发核心

• 批准号: 10747628
• 负责人: Gerard L Apodaca
• 金额: $ 23.17万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10747628
• 关键词: 3-Dimensional; 4D Imaging; Address; Antibodies; Antigens; Architecture; Basic Science; Binding; Biological; Biological Models; Cell physiology; Cells; Collaborations; Collagen; Collection; Computers; Country; Creativeness; Cues; Custom; Dedications; Development; Disease; Educational process of instructing; Electron Microscope; Electrons; Energy Transfer; Ensure; Equipment; Faculty; Fluorochrome; Freeze Fracturing; Freeze Substitution; Functional disorder; Funding; Goals; Gold; Hand; Image; Imaging Device; Imaging technology; Incubators; Individual; Industry; Infrastructure; Injury; Kidney; Label; Light; Location; Lower urinary tract; Maintenance; Methods; Microfluidics; Microscope; Microscopy; Mission; Organ; Organelles; Organoids; Performance; Physiology; Polarization Microscopy; Positioning Attribute; Protocols documentation; Quality Control; Reagent; Recording of previous events; Renal function; Research; Research Personnel; Resource Development; Resources; Scanning; Schedule; Site; Standardization; Structure; System; Technology; Technology Transfer; Testing; Three-Dimensional Imaging; Time; Tissues; Training; Translational Research; Urinary tract; Validation; Variant; Vertebral column; Work; base; cell type; cohort; cost; design; extracellular; image processing; imaging facilities; imaging modality; imaging study; imaging system; improved; innovation; kidney cell; kidney dysfunction; kidney imaging; manufacture; mechanotransduction; microscopic imaging; multidisciplinary; novel; petabyte; repaired; response; screening; second harmonic; skills; superresolution imaging; technology development; technology platform; terabyte; tool; tool development; transmission process; two-photon; ultra high resolution; virtual reality

ABSTRACT: KIDNIT (Kidney Imaging: Developing Novel and Innovative Tools) is the Pittsburgh Center for Kidney Research’s Resource Development Core. Working in collaboration with kidney investigators and the Center’s Physiology and Model Systems Core, KIDNIT will build and adapt cutting-edge light, super-resolution, and electron microscopic imaging modalities and technologies to address open questions related to kidney function and dysfunction. The broad and long-range goals of KIDNIT are as follows: Aim 1 is to nurture a dynamic incubator space for imaging tool development. KIDNIT will capitalize on the resources provided by the Center for Biological Imaging (CBI), one of the largest imaging facilities in the country. The CBI has a dedicated expert faculty, a large cohort of research technicians, and a vast array of imaging equipment including over 40 contemporary widefield, confocal, two-photon, TIRF, light-sheet, super-resolution, and electron microscopes. Aim 2 is to develop new and custom-made imaging resources, tools, and technologies specifically to augment and advance kidney research. KIDNIT will leverage the CBI’s faculty expertise to develop, adapt, and implement new imaging tools for kidney researchers. Example projects in development include novel clearing and 3D imaging protocols to reveal and quantify kidney architecture and function at the level of the entire organ; analyzing collagen organization and structure in normal and fibrotic tissues using a recently developed variant of polarized light microscopy (instant polarized light microscopy; iPOL); development of new protocols that employ fluorescent lifetime imaging and fluorescent energy transfer to rapidly assess kidney cell function and responses to extracellular cues; use of high throughput platforms to perform large-scale screening of organoid form and function; and development of novel microfluidic chambers. Mechanisms are in place to ensure training and information transfer. Aim 3 is focused on ensuring robust validation and quality control of new resources. An example is a validated antibody collection that can be used to assess differentiation, injury, and repair status of kidney cells and tissues. Aim 4 is to collaborate with the Consortium to prioritize, suspend, or implement new and improved imaging resources. Working in collaboration with the Consortium, KIDNIT will replace obsolete technologies by leveraging its ties with industry leaders, along with a strong track record of S10 funding, to offer the most recent developments in microscopy, specifically to advance the research of our investigator collective. Impact: KIDNIT will advance kidney research by providing Center and Consortium investigators with novel and bespoke imaging tools that will provide critical information about the location, dynamics, amounts, and function of kidney-associated molecules, organelles, cells, and tissues in normal and disease states.

摘要：KIDNIT（肾脏成像：开发新颖和创新工具）是匹兹堡中心 肾脏研究的资源开发核心。与肾脏研究人员和 作为该中心的生理学和模型系统核心，KIDNIT 将构建和采用尖端的光、超分辨率、 和电子显微成像模式和技术来解决与肾脏相关的悬而未决的问题 功能和功能障碍。 KIDNIT 的广泛而长期的目标如下： 目标 1 是培养充满活力的 成像工具开发的孵化空间。 KIDNIT 将利用该中心提供的资源 生物成像 (CBI)，该国最大的成像设施之一。 CBI 有专门的专家 教职员工、大批研究技术人员和大量成像设备，其中包括 40 多台 现代宽场、共焦、双光子、TIRF、光片、超分辨率和电子显微镜。 目标 2 是开发新的定制成像资源、工具和技术，专门用于增强 并推进肾脏研究。 KIDNIT 将利用 CBI 教师的专业知识来开发、调整和实施 为肾脏研究人员提供的新成像工具。正在开发的示例项目包括新颖的清理和 3D 成像协议以揭示和量化整个器官水平的肾脏结构和功能； 使用最近开发的变体分析正常和纤维化组织中的胶原蛋白组织和结构 偏光显微镜（即时偏光显微镜；iPOL）；开发新协议 采用荧光寿命成像和荧光能量转移来快速评估肾细胞功能和 对细胞外信号的反应；使用高通量平台进行类器官的大规模筛选 形式和功能；和新型微流体室的开发。确保培训的机制已到位 和信息传递。目标 3 侧重于确保新资源的稳健验证和质量控制。 一个例子是经过验证的抗体集合，可用于评估分化、损伤和修复状态 肾细胞和组织。目标 4 是与联盟合作确定优先顺序、暂停或实施新的 并改进了成像资源。与该联盟合作，KIDNIT 将取代过时的 通过利用与行业领导者的联系以及 S10 融资的良好记录， 显微镜的最新发展，特别是为了推进我们研究人员集体的研究。 影响：KIDNIT 将通过为中心和联盟研究人员提供新颖且可靠的方法来推进肾脏研究 定制成像工具将提供有关位置、动态、数量和功能的关键信息 正常和疾病状态下的肾脏相关分子、细胞器、细胞和组织。