Resource Development Core

摘要：KIDNIT（肾脏成像：开发新颖和创新的工具）是匹兹堡中心， 肾脏研究的资源开发核心。与肾脏研究人员和 中心的生理学和模型系统核心，KIDNIT将建立和适应尖端的光，超分辨率， 和电子显微镜成像模式和技术，以解决与肾脏相关的未决问题， 功能和功能障碍。KIDNIT的广泛和长期目标如下：目标1是培养一个充满活力的 用于成像工具开发的孵化器空间。KIDNIT将利用该中心提供的资源 生物成像（CBI），在该国最大的成像设施之一。加调局有专门的专家 教师，研究技术人员的一大群，以及大量的成像设备，包括40多个 当代宽视场、共焦、双光子、TIRF、光片、超分辨率和电子显微镜。 目标2是开发新的和定制的成像资源、工具和技术， 推进肾脏研究KIDNIT将利用CBI的教师专业知识来开发，适应和实施 肾脏研究人员的新成像工具。开发中的示例项目包括新型清算和3D 成像协议，以揭示和量化肾脏结构和功能在整个器官的水平; 使用最近开发的变体分析正常和纤维化组织中的胶原组织和结构 偏振光显微镜（即时偏振光显微镜; iPOL）;开发新的协议， 使用荧光寿命成像和荧光能量转移来快速评估肾细胞功能， 对细胞外信号的反应;使用高通量平台进行类器官的大规模筛选 形式和功能;以及新型微流体腔室的开发。建立了确保培训的机制 和信息传输。目标3的重点是确保对新资源进行可靠的验证和质量控制。 一个例子是经过验证的抗体收集，可用于评估分化，损伤和修复状态 肾脏细胞和组织。目标4是与联盟合作，优先考虑、暂停或实施新的 和改进的成像资源。KIDNIT将与联合会合作， 利用其与行业领导者的关系，沿着S10融资的良好记录， 显微镜的最新发展，特别是推进我们的研究集体。 影响：KIDNIT将通过为中心和联盟研究人员提供新的， 定制的成像工具，将提供有关位置，动态，数量和功能的关键信息 肾脏相关分子，细胞器，细胞和组织在正常和疾病状态。

ABSTRACT: KIDNIT (Kidney Imaging: Developing Novel and Innovative Tools) is the Pittsburgh Center for Kidney Research’s Resource Development Core. Working in collaboration with kidney investigators and the Center’s Physiology and Model Systems Core, KIDNIT will build and adapt cutting-edge light, super-resolution, and electron microscopic imaging modalities and technologies to address open questions related to kidney function and dysfunction. The broad and long-range goals of KIDNIT are as follows: Aim 1 is to nurture a dynamic incubator space for imaging tool development. KIDNIT will capitalize on the resources provided by the Center for Biological Imaging (CBI), one of the largest imaging facilities in the country. The CBI has a dedicated expert faculty, a large cohort of research technicians, and a vast array of imaging equipment including over 40 contemporary widefield, confocal, two-photon, TIRF, light-sheet, super-resolution, and electron microscopes. Aim 2 is to develop new and custom-made imaging resources, tools, and technologies specifically to augment and advance kidney research. KIDNIT will leverage the CBI’s faculty expertise to develop, adapt, and implement new imaging tools for kidney researchers. Example projects in development include novel clearing and 3D imaging protocols to reveal and quantify kidney architecture and function at the level of the entire organ; analyzing collagen organization and structure in normal and fibrotic tissues using a recently developed variant of polarized light microscopy (instant polarized light microscopy; iPOL); development of new protocols that employ fluorescent lifetime imaging and fluorescent energy transfer to rapidly assess kidney cell function and responses to extracellular cues; use of high throughput platforms to perform large-scale screening of organoid form and function; and development of novel microfluidic chambers. Mechanisms are in place to ensure training and information transfer. Aim 3 is focused on ensuring robust validation and quality control of new resources. An example is a validated antibody collection that can be used to assess differentiation, injury, and repair status of kidney cells and tissues. Aim 4 is to collaborate with the Consortium to prioritize, suspend, or implement new and improved imaging resources. Working in collaboration with the Consortium, KIDNIT will replace obsolete technologies by leveraging its ties with industry leaders, along with a strong track record of S10 funding, to offer the most recent developments in microscopy, specifically to advance the research of our investigator collective. Impact: KIDNIT will advance kidney research by providing Center and Consortium investigators with novel and bespoke imaging tools that will provide critical information about the location, dynamics, amounts, and function of kidney-associated molecules, organelles, cells, and tissues in normal and disease states.