A Compact X-ray Station for Protein Crystallography

用于蛋白质晶体学的紧凑型 X 射线站

• 批准号: 7911573
• 负责人: JEFFREY RIFKIN
• 金额: $ 118.44万
• 依托单位: LYNCEAN TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7911573
• 关键词: Capital; Communities; Crystallography; Development; Elements; Engineering; Equipment; Grant; Home environment; Institution; Intercept; Investments; Laboratories; Light; Mechanics; Motion; National Institute of General Medical Sciences; Operative Surgical Procedures; Optics; Output; Performance; Phase; Principal Investigator; Productivity; Protein Structure Initiative; Proteins; Proteomics; Research; Resolution; Resources; Roentgen Rays; Running; Screening procedure; Services; Side; Small Business Innovation Research Grant; Source; Spottings; Structure; Synchrotrons; System; Systems Integration; Testing; base; beamline; cost; design; drug discovery; engineering design; innovation; instrument; programs; prototype; research study; structural biology; structural genomics

DESCRIPTION (provided by applicant): The purpose of this Fast-Track SBIR is to develop an innovative Compact X-ray Station (CXS) for macromolecular crystallography. This station accepts the output x-ray beam of the Compact Light Source, a miniature synchrotron, and delivers x-ray beams to 3 end stations. Each beamline accommodates an integrated commercial diffractometer. Each of 2 side stations receives a tunable, monochromatic x-ray beam in a 30 micron rms spot. The forward station receives a focused higher-flux narrow band x-ray beam. Each of the x-ray beams is tunable from 7 to 16 keV and will provide a flux and energy resolution comparable to synchrotron beamlines. The beamlines can be run separately, in pairs or all 3 simultaneously. In Phase I, we will design the x-ray optics systems and experimentally test a new, tunable focusing monochromator. In Phase II, we will build the x-ray optics and end stations, integrate a commercial diffractometer and fully test the end stations. The CXS is an innovative, powerful, 'turn key' x-ray system which will provide a home laboratory with 3 'synchrotron' beamlines for macromolecular crystallography; 2 beamlines will have the flux, tunability, and energy resolution for single- or multi-wavelength anomalous dispersion experiments, and the third beamline will be optimized for higher-flux screening.

描述(申请人提供)：此快速跟踪SBIR的目的是开发一种创新的紧凑型X射线站(CXS)，用于大分子结晶学。该站接受小型同步加速器紧凑型光源的输出X射线束，并向3个终端站提供X射线束。每条光束线都配有一个集成的商用衍射仪。两个侧站中的每一个都接收30微米RMS光斑中的可调谐单色X射线束。前置站接收聚焦的高通量窄带X射线束。每一束X射线都可以在7到16 keV范围内调谐，并将提供可与同步加速器光束线相媲美的通量和能量分辨率。光束线可以单独运行、成对运行或同时运行所有3个。在第一阶段，我们将设计X射线光学系统，并实验测试一种新型的可调聚焦单色器。在第二阶段，我们将建造X射线光学系统和终端站，集成商用衍射仪，并对终端站进行全面测试。CXS是一种创新、强大的“交钥匙”X射线系统，它将为家庭实验室提供3条用于大分子结晶学的“同步加速器”光束线；2条光束线将具有用于单波长或多波长反常色散实验的通量、可调性和能量分辨率；第三条光束线将进行优化，以进行更高通量的筛选。

项目成果

Comparison of contrast-to-noise ratios of transmission and dark-field signal in grating-based X-ray imaging for healthy murine lung tissue.

• DOI: 10.1016/j.zemedi.2012.11.003
• 发表时间: 2013-09
• 期刊: Zeitschrift fur medizinische Physik
• 影响因子: 2
• 作者: F. Schwab;S. Schleede;D. Hahn;M. Bech;J. Herzen;S. Auweter;F. Bamberg;K. Achterhold;A. Yildirim;A. Bohla;O. Eickelberg;R. Loewen;M. Gifford;R. Ruth;M. Reiser;K. Nikolaou;F. Pfeiffer;F. Meinel