CORE C MOUSE TRANSGENIC AND MAGNETIC RESONANCE IMAGING CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该核心的目标是提供设施和技术专长，以支持涉及小鼠模型的项目，作为解决重要生物学和生物医学问题的一种手段。核心的服务包括通过原核注射产生转基因小鼠，胚胎干细胞（ES）细胞的基因靶向，ES细胞注射到囊胚中，再衍生，小鼠胚胎冷冻保存，以及共享小鼠品系的进口，维护和分销，以及小动物磁共振成像。今年的新组件包括扩展的体内成像能力，具有高分辨率超声和microCT组件。小鼠转基因组件/microCT由Lucy Liaw，Ph.D.监督，并雇佣了两名全职研究助理磁共振成像和超声组件由Ilka Pinz博士指导，并聘请一名全职研究助理。这个核心设施已经为我们研究所的所有研究人员提供服务，并将转基因和成像服务扩展到COBRE州的研究人员和新英格兰地区的其他研究人员。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objectives of this Core are to provide facilities and technical expertise in support of projects involving mouse models as a means of addressing important biological and biomedical questions. Services of the core include generation of transgenic mice by pronuclear injection, gene targeting in embryonic stem (ES) cells, ES cell injection into blastocysts, re-derivation, mouse embryo cryopreservation, and the importation, maintenance, distribution of shared mouse strains, and small animal magnetic resonance imaging. New components for this year include expanded in vivo imaging capacity with a high resolution ultrasound and a microCT component. The Mouse Transgenic components/microCT are overseen by Lucy Liaw, Ph.D., and employs two full-time research associates. The magnetic resonance imaging and the ultrasound components are directed by Ilka Pinz, Ph.D., and employ one full-time research assistant. This core facility has served all investigators within our Institute, and has extended transgenic and imaging services to investigators in COBRE states and other investigators in the New England region.