Rapid Multipathogen Detection for POCT and National Diaster Readiness

描述（由申请人提供）： UC Davis-Lawrence Livermore国家实验室（LLNL）护理点检测（POCT）中心的目标是提高POC设备在重症监护室、急诊室、社区医院、农村地区和灾难响应现场的可访问性、便携性和现场鲁棒性。 核心1。LLNL团队将开发一种紧凑的用户友好型POC系统，该系统使用等温环介导扩增（LAMP）同时检测多种病原体（念珠菌属，E.柯尔特假单胞菌葡萄球菌金黄色葡萄球菌和链球菌。在人类全血样本中，减少到实践中作为一个原型的医院设置和第二个更强大的原型，在紧急情况和灾害中快速现场诊断。 核心2。UCD团队将测试核心1原型，并促进新的POC设备的开发与关键紧急灾难护理的整体主题一致，并作为新兴POC技术评估的网络资源，以定义热，冷，湿度，冲击和水暴露的环境耐受极限（例如，卡特里娜飓风）。 核心3。早期的实际试验和需求评估调查将为灾害管理援助队、移动的救援单位和小世界网络中的POCT实施产生稳健性标准和循证标准;全球基于网络的远程学习和研讨会将教育、培训和传播我们新的最先进的教育大楼和William F.布莱斯德尔医学图书馆在UCDMC。 核心4。POCT中心将培训医疗保健和技术开发人员快速病原体检测的临床应用和灾难中发现的不利环境的POCT实践。 核心5。综合领导将使POCT的发现迅速进入日常医疗实践。在UCDMC和加州大学圣地亚哥分校的灾难医疗响应无线互联网信息系统中进行的POC连接试验将为紧急情况部署POCT。UCD-LLNL中心将通过开发新的POC设备来促进公共卫生，这些设备可以加速对危及生命的血流感染的诊断和治疗，为我们的国家应对未来的灾难做好准备，并通过在医院床边的快速决策来提高患者的生存率和结果，在急诊室的急性护理期间，以及灾难发生时的现场救援。

DESCRIPTION (provided by applicant): The goal of the UC Davis-Lawrence Livermore National Laboratory (LLNL) point-of-care testing (POCT) Center is to improve the accessibility, portability, and field robustness of POC devices for critical care units, emergency rooms, community hospitals, rural areas, and disaster response sites. Core 1. The LLNL team will develop a compact user-friendly POC system that uses isothermal loop mediated amplification (LAMP) for simultaneous detection of multiple pathogens (Candida sp., E. Colt, Pseudomonas, Staph. Aureus, and Strep. Pneumoniae) in human whole-blood samples, with reduction to practice as one prototype for hospital settings and a second more robust prototype for rapid field diagnosis in emergencies and disasters. Core 2. The UCD team will test the Core 1 prototypes and facilitate the development of new POC devices consistent with the overall theme of critical-emergencv-disaster care and also serve as a Network resource for the evaluation of emerging POC technologies to define environmental tolerance limits for heat, cold, humidity, shock, and water exposure (e.g., Hurricane Katrina). Core 3. Early practical trials and needs assessment surveys will generate robustness criteria and evidence-based standards for POCT implementation in DMATs (Disaster Management Assistance Teams), mobile rescue units, and small-world networks; worldwide web-based distance learning and workshops will educate, train, and disseminate vital information from our new state-of-the-art Education Building and William F. Blaisdell Medical Library at UCDMC. Core 4. The POCT Center will train healthcare and technical developers in clinical applications of rapid pathogen detection and in POCT practices for adverse environments found in disasters. Core 5. Integrative leadership will move POCT discoveries swiftly into daily medical practice. POC connectivity trials with Telemedicine at UCDMC and the Wireless Internet Information System for Medical Response in Disasters, UC San Diego, will deploy POCT for emergencies. The UCD-LLNL Center will advance public health by developing new POC devices that accelerate diagnosis and treatment of life-threatening bloodstream infections, prepare our nation for future disasters, and enhance patient survival and outcomes through rapid decision making at the bedside in hospitals, during acute care in emergency rooms, and for field rescue when disasters strike.