Rapid Multipathogen Detection for POCT and National Diaster Readiness

描述(由申请人提供)： 加州大学戴维斯分校-劳伦斯利弗莫尔国家实验室(LLNL)护理点检测(POCT)中心的目标是提高重症监护病房、急诊室、社区医院、农村地区和灾难应对站点的POC设备的可及性、便携性和现场稳定性。 LLNL团队将开发一种紧凑、用户友好的POC系统，该系统使用等温环介导扩增(LAMP)同时检测多种病原体(念珠菌、E.Colt、假单胞菌、葡萄球菌)。金星和链霉菌。人类全血样本中的肺炎(肺炎)，作为医院环境下的一个原型进行实践，以及在紧急情况和灾难中用于快速现场诊断的第二个更强大的原型。 核心2.UCD团队将测试Core 1原型，促进符合危急-紧急-灾难护理总体主题的新PoC设备的开发，并作为评估新兴PoC技术的网络资源，以确定热、冷、湿度、冲击和水暴露(例如，卡特里娜飓风)的环境容忍度限制。 早期实践试验和需求评估调查将为灾难管理援助小组(DMATs)、移动救援单位和小世界网络中POCT的实施产生稳健性标准和基于证据的标准；全球基于网络的远程学习和研讨会将教育、培训和传播我们新的最先进的教育大楼和UCDMC的William F.Blaisdell医学图书馆的重要信息。 核心4.POCT中心将就快速病原体检测的临床应用和灾难中发现的不利环境的POCT实践对医疗保健和技术开发人员进行培训。 综合领导力将迅速将POCT的发现转化为日常医疗实践。UCDMC和加州大学圣地亚哥分校灾难中医疗响应无线互联网信息系统的PoC连接试验将在紧急情况下部署POCT。

DESCRIPTION (provided by applicant): The goal of the UC Davis-Lawrence Livermore National Laboratory (LLNL) point-of-care testing (POCT) Center is to improve the accessibility, portability, and field robustness of POC devices for critical care units, emergency rooms, community hospitals, rural areas, and disaster response sites. Core 1. The LLNL team will develop a compact user-friendly POC system that uses isothermal loop mediated amplification (LAMP) for simultaneous detection of multiple pathogens (Candida sp., E. Colt, Pseudomonas, Staph. Aureus, and Strep. Pneumoniae) in human whole-blood samples, with reduction to practice as one prototype for hospital settings and a second more robust prototype for rapid field diagnosis in emergencies and disasters. Core 2. The UCD team will test the Core 1 prototypes and facilitate the development of new POC devices consistent with the overall theme of critical-emergencv-disaster care and also serve as a Network resource for the evaluation of emerging POC technologies to define environmental tolerance limits for heat, cold, humidity, shock, and water exposure (e.g., Hurricane Katrina). Core 3. Early practical trials and needs assessment surveys will generate robustness criteria and evidence-based standards for POCT implementation in DMATs (Disaster Management Assistance Teams), mobile rescue units, and small-world networks; worldwide web-based distance learning and workshops will educate, train, and disseminate vital information from our new state-of-the-art Education Building and William F. Blaisdell Medical Library at UCDMC. Core 4. The POCT Center will train healthcare and technical developers in clinical applications of rapid pathogen detection and in POCT practices for adverse environments found in disasters. Core 5. Integrative leadership will move POCT discoveries swiftly into daily medical practice. POC connectivity trials with Telemedicine at UCDMC and the Wireless Internet Information System for Medical Response in Disasters, UC San Diego, will deploy POCT for emergencies.